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Titolo:
Specific pancreatic enzymes activate macrophages to produce tumor necrosisfactor-alpha: Role of nuclear factor kappa B and inhibitory kappa B proteins
Autore:
Jaffray, C; Mendez, C; Denham, W; Carter, G; Norman, J;
Indirizzi:
Univ S Florida, Dept Surg, Tampa Gen Hosp, Tampa, FL USA Univ S Florida Tampa FL USA da, Dept Surg, Tampa Gen Hosp, Tampa, FL USA
Titolo Testata:
JOURNAL OF GASTROINTESTINAL SURGERY
fascicolo: 4, volume: 4, anno: 2000,
pagine: 370 - 377
SICI:
1091-255X(200007/08)4:4<370:SPEAMT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LUNG INJURY; ALVEOLAR MACROPHAGES; MANNOSE RECEPTOR; CELL-LINE; INTERLEUKIN-1; MECHANISM; CYTOKINES; PATHWAYS;
Keywords:
pancreatitis; enzyme; macrophage; NF-kappa B; I kappa B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Norman, J Univ S Florida, Dept Surg, Tampa Gen Hosp, POB 1289, Tampa, FL USA Univ S Florida POB 1289 Tampa FL USA p, POB 1289, Tampa, FL USA
Citazione:
C. Jaffray et al., "Specific pancreatic enzymes activate macrophages to produce tumor necrosisfactor-alpha: Role of nuclear factor kappa B and inhibitory kappa B proteins", J GASTRO S, 4(4), 2000, pp. 370-377

Abstract

The triggering events hv which mononuclear cells throughout the body are induced to produce large amounts of cytokines during acute pancreatitis are unclear. However; recent work in our laboratory demonstrated that three specific pancreatic enzymes (elastase, carboxypeptidase A, and lipase) induceddramatic tumor necrosis factor-alpha (TNF-alpha) protein production from macrophages, whereas all others could not. This series of experiments was designed to examine the second messenger system by which this occurs. The ratmacrophage cell line NR8383 was incubated for 3 hours with elastase, carboxypeptidase A, lipase, trypsin, or lipopolysaccharide (positive control). Activation of nuclear factor kappa B (NF-kappa B) was demonstrated by electrophoretic mobility shift assay, presence of inhibitory kappa B alpha and beta (I kappa B-alpha and I kappa B-beta) by Western blot analysis, and TNF-alpha protein production by enzyme-linked immunosorbent assay. Elastase, carboxypeptidase A, and lipase induced degradation of I kappa B-beta (but not I kappa B-alpha), activation of NF-kappa B, and production of TNF-alpha protein, whereas inhibition of I kappa B with pyrrolidine dithiocarbamate attenuated this response. Trypsin was unable to elicit any of these responses. Macrophages can be induced by specific activated pancreatic enzymes-elastase, carboxypeptidase A and lipase-to produce TNF-alpha. This process is dependent on I kappa B-beta degradation and NF-kappa B activation, suggesting that these enzymes trigger this second messenger system through specific membrane-bound receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:21:05