Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ras mediates effector pathways responsible for pre-B cell survival, which is essential for the developmental progression to the late pre-B cell stage
Autore:
Nagaoka, H; Takahashi, Y; Hayashi, R; Nakamura, T; Ishii, K; Matsuda, J; Ogura, A; Shirakata, Y; Karasuyama, H; Sudo, T; Nishikawa, S; Tsubata, T; Mizuochi, T; Asano, T; Sakano, H; Takemori, T;
Indirizzi:
Natl Inst Infect Dis, Dept Immunol, Shinjuku Ku, Tokyo 1628640, Japan NatlInst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis, Dept Vet Sci, Shinjuku Ku, Tokyo 1628640, Japan NatlInst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis, Div Expt Anim Res, Shinjuku Ku, Tokyo 1628640, JapanNatl Inst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Univ Tokyo, Grad Sch Sci, Dept Biochem & Biophys, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 t Biochem & Biophys, Tokyo 1130033, Japan Tokai Univ, Dept Appl Chem, Lab Biomed Chem, Kanagawa 2591292, Japan TokaiUniv Kanagawa Japan 2591292 b Biomed Chem, Kanagawa 2591292, Japan Japanese Fdn Canc Res, Inst Canc, Dept Gene Res, Tokyo 1708455, Japan Japanese Fdn Canc Res Tokyo Japan 1708455 Gene Res, Tokyo 1708455, Japan Tokyo Metropolitan Inst Med Sci, Dept Immunol, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613, Japan Toray Basic Res Labs, Kanagawa 2591192, Japan Toray Basic Res Labs Kanagawa Japan 2591192 abs, Kanagawa 2591192, Japan Kyoto Univ, Grad Sch Med, Dept Mol Genet, Kyoto 6068507, Japan Kyoto UnivKyoto Japan 6068507 Med, Dept Mol Genet, Kyoto 6068507, Japan Tokyo Med & Dent Univ, Med Res Inst, Dept Immunol, Tokyo 1138510, Japan Tokyo Med & Dent Univ Tokyo Japan 1138510 Immunol, Tokyo 1138510, Japan
Titolo Testata:
JOURNAL OF EXPERIMENTAL MEDICINE
fascicolo: 2, volume: 192, anno: 2000,
pagine: 171 - 181
SICI:
0022-1007(20000717)192:2<171:RMEPRF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURROGATE LIGHT-CHAIN; MOUSE BONE-MARROW; ALLELIC EXCLUSION; PHOSPHOINOSITIDE 3-KINASE; GENE REARRANGEMENT; IG-BETA; MICE; EXPRESSION; PROTEIN; ACTIVATION;
Keywords:
B cell development; life span; immunoglobulin gene rearrangement; Bcl-xL; Bcl-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Takemori, T Natl Inst Infect Dis, Dept Immunol, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628640, Japan Natl Inst Infect Dis 1-23-1 Toyama Tokyo Japan 1628640 Japan
Citazione:
H. Nagaoka et al., "Ras mediates effector pathways responsible for pre-B cell survival, which is essential for the developmental progression to the late pre-B cell stage", J EXP MED, 192(2), 2000, pp. 171-181

Abstract

Ras is essential for the transition from early B cell precursors to the pro-B stage, and is considered to be involved in the signal cascade mediated by pre-B cell antigen receptors. To examine the role of p21(ras) in the late stage of B cell differentiation, we established transgenic mice (TG) expressing a dominant-inhibitory mutant of Ha-ras (Asn-17 Ha-ras) in B lineage cells at high levels after the early B cell precursor stage. Expression of p21(Asn-17) (Ha-ras) was associated with a prominent reduction in the number of late pre-B cells, but had little effect on proliferation of early pre-B cells. inhibition of p21(ras) activity markedly reduced the life span of pre-B cells, due, at least in part, to downregulation of the expression of an antiapoptotic protein, Bcl-xL. Thus, the apparent role for p21(ras) activity in pre-B cell survival may explain the decreased numbers of late pre-Bcells in Asn-17 Ha-ras TG. Consistent with this possibility, overexpression of Bcl-2 in Asn-17 (Ha-ras) TG reversed the reduction in the number of late pre-B cells undergoing immunoglobulin light chain gene (IgL) rearrangement and progressing to immature B cells. These results suggest that p21(ras)mediates effector pathways responsible for pre-B cell survival, which is essential for progression to the late pre-B and immature B stages.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 09:35:00