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Titolo:
Changes in the surface structure of the hamster sperm head associated withmaturation, in vitro capacitation and acrosome reaction: an atomic force microscopic study
Autore:
Takano, H; Abe, K;
Indirizzi:
Hokkaido Univ, Grad Sch Med, Dept Anat, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan
Titolo Testata:
JOURNAL OF ELECTRON MICROSCOPY
fascicolo: 3, volume: 49, anno: 2000,
pagine: 437 - 443
SICI:
0022-0744(2000)49:3<437:CITSSO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FILIPIN-STEROL COMPLEXES; MONOCLONAL-ANTIBODIES; INVITRO CAPACITATION; PLASMA-MEMBRANE; INTRAMEMBRANOUS PARTICLES; EPIDIDYMAL MATURATION; RAT SPERMATOZOA; ANTIGENS; FERTILIZATION; LOCALIZATION;
Keywords:
surface structure; sperm head; hamster; maturation; capacitation; acrosome reaction; atomic force microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Takano, H Hokkaido Univ, Grad Sch Med, Dept Anat, Kita Ku, Kita 15 Nishi 7, Sapporo,Hokkaido 0608638, Japan Hokkaido Univ Kita 15 Nishi 7 Sapporo Hokkaido Japan 0608638 pan
Citazione:
H. Takano e K. Abe, "Changes in the surface structure of the hamster sperm head associated withmaturation, in vitro capacitation and acrosome reaction: an atomic force microscopic study", J ELEC MICR, 49(3), 2000, pp. 437-443

Abstract

Structural changes of the hamster sperm head surface associated with maturation, capacitation and acrosome reaction were examined by atomic force microscopy. Spermatozoa were taken from the initial segment. and distal cauda of the epididymis, washed in a modified Tyrode solution and fixed by glutaraldehyde. Some sperms taken from distal cauda epididymides were incubated with the capacitation medium before fixation. All samples were attached on the glass slide, dried in a critical point drier and observed by atomic force microscopy. The sperm head surface was characterized by the presence of numerous round particles, similar to 40 and 60 nm in diameter. The distribution and density of these particles on the sperm surface were significantly different between the equatorial segment and post-acrosomal region in each sperm, and also between sperms under different conditions. The surface of the equatorial segment was rather smooth in sperms from the initial segment of the epididymis, but: had many large (60 nm) particles in sperms from thedistal cauda epididymides, suggesting that the large particles were glycoproteins which were secreted from the epididymis and attached to the sperm surface during maturation. The number of these particles dramatically decreased in both capacitated acrosome-unreacted and acrosome-reacted sperms. This finding supports the idea that glycoproteins are removed from the sperm surface during capacitation. Atomic force microscopic studies of the sperm head surface are expected to be used for future molecular studies on the cell surface components involved in the mechanism of maturation, capacitation and acrosome reaction.

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Documento generato il 29/11/20 alle ore 02:51:52