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Titolo:
Misdirected vimentin messenger RNA alters cell morphology and motility
Autore:
Morris, EJ; Evason, K; Wiand, C; LEcuyer, TJ; Fulton, AB;
Indirizzi:
Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 a, Dept Biochem, Iowa City, IA 52242 USA Wayne State Univ, Sch Med, Childrens Hosp Michigan, Dept Pediat, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 Dept Pediat, Detroit, MI 48201 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 13, volume: 113, anno: 2000,
pagine: 2433 - 2443
SICI:
0021-9533(200007)113:13<2433:MVMRAC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; BETA-ACTIN; INTRACELLULAR-LOCALIZATION; 3'-UNTRANSLATED REGION; CYTOSKELETAL PROTEINS; SKELETAL-MUSCLE; TROPOMYOSIN; EXPRESSION; POLARITY; CHICKEN;
Keywords:
vimentin; mRNA location; cell shape; cell movement;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Fulton, AB Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 chem, Iowa City, IA 52242 USA
Citazione:
E.J. Morris et al., "Misdirected vimentin messenger RNA alters cell morphology and motility", J CELL SCI, 113(13), 2000, pp. 2433-2443

Abstract

Localized messenger RNAs were first observed as embryonic determinants that altered development when mislocalized. In recent years localized mRNAs have been found for several cytoskeletal proteins, including actin, vimentin and several microtubule associated proteins, We sought to determine whetherredirecting mRNA for a cytoskeletal protein to an inappropriate address would alter cellular phenotypes. To do so we generated vimentin mRNAs with a myc epitope tag and the beta-actin 3' untranslated region (3' UTR) as a localization signal. When misdirected vimentin mRNAs are expressed in either fibroblasts or SW13 cells, cells develop numerous, extremely long processes;these cells also move more slowly to enter a wound of the monolayer. In situ hybridization revealed that the misdirected mRNA was often localized in the processes, in contrast to endogenous vimentin mRNA, The processes usually contained actin distal to the transgenic vimentin and microtubules proximal to it, SW13 cells lacking vimentin produced fewer and shorter processes, suggesting a dominant negative effect that involves recruitment of endogenous vimentin. Control experiments that transfected in constructs expressing tagged, correctly localized vimentin, or beta-galactosidase that localized through the beta-actin 3' UTR, indicate that neither the shape nor the motility changes are solely due to the level of vimentin expression in the cell, This is direct evidence that the site of expression for at least one cytoskeletal mRNA alters the phenotype of the cell in which it is expressed, Messenger RNA localization is proving to be as essential for the normal maintenance of somatic cell phenotypes as embryonic determinants are for embryogenesis.

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Documento generato il 26/09/20 alle ore 13:47:26