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Titolo:
Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells
Autore:
Abe, Y; Matsumoto, S; Kito, K; Ueda, N;
Indirizzi:
Ehime Univ, Sch Med, Dept Pathol 1, Shigenobu, Ehime 7910295, Japan Ehime Univ Shigenobu Ehime Japan 7910295 Shigenobu, Ehime 7910295, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 28, volume: 275, anno: 2000,
pagine: 21525 - 21531
SICI:
0021-9258(20000714)275:28<21525:CAEOAN>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNALING PATHWAY; KILLING ACTIVITY; LAK CELLS; LYMPHOTOXIN; PROLIFERATION; INTERLEUKIN-2; COMPLEX; CASCADE; MEMBER; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Abe, Y Ehime Univ, Sch Med, Dept Pathol 1, Shigenobu, Ehime 7910295, JapanEhime Univ Shigenobu Ehime Japan 7910295 obu, Ehime 7910295, Japan
Citazione:
Y. Abe et al., "Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells", J BIOL CHEM, 275(28), 2000, pp. 21525-21531

Abstract

A novel protein kinase, TOPK (T-LAK cell-originated protein kinase), was isolated from a lymphokine-activated killer T (T-LAK) cell subtraction cDNA fragment library. The open reading frame of the TOPK gene encodes a proteinof 322 amino acids, possessing a protein kinase domain profile. The cap site analysis of the 5'-end of TOPK mRNA revealed two forms, a major full-length form and a minor spliced form at the 5'-site, both encoding the same protein. A BLAST homology search and phylogenetic analysis indicated that TOPK is related to dual specific mitogen-activated protein kinase kinase (MAPKK). The transfection of the TOPK gene to COS-7 cells up-regulated a phosphorylation of p38 MAPK but not ERK1/2 or SAPK/JNK. Gel precipitation study indicated that TOPK protein can be associated with p38 in vitro. Tissue distribution of TOPK mRNA expression was specific for the testis, T-LAK cells, activated lymphoid cells, and lymphoid tumors. On the other hand, deactivated T-LAK, cells did not show TOPK mRNA expression. These data suggest that TOPK is a newly identified member of a novel MEK3/6-related MAPKK that may be enrolled in the activation of lymphoid cells and support testicular functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:45:32