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Titolo:
gamma-radiation-induced single cell DNA damage as a measure of susceptibility to lung cancer: A preliminary report
Autore:
Zhang, HF; Buchholz, TA; Hancock, D; Spitz, MR; Wu, XF;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Epidemiol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Epidemiol, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Radiat Oncol, Houston, TX 77030 USAUniv Texas Houston TX USA 77030 Dept Radiat Oncol, Houston, TX 77030 USA Univ Texas, Sch Publ Hlth, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 xas, Sch Publ Hlth, Houston, TX 77030 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 2, volume: 17, anno: 2000,
pagine: 399 - 404
SICI:
1019-6439(200008)17:2<399:GSCDDA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
UPPER AERODIGESTIVE TRACT; BENZOPYRENE DIOL EPOXIDE; DOUBLE-STRAND BREAKS; MUTAGEN SENSITIVITY; GEL-ELECTROPHORESIS; INDIVIDUAL CELLS; COMET ASSAY; RISK FACTOR; REPAIR; BLEOMYCIN;
Keywords:
comet assay; susceptibility; lung cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, XF Univ Texas, MD Anderson Canc Ctr, Dept Epidemiol, 1515 Holcombe Blvd,Box 189, Houston, TX 77030 USA Univ Texas 1515 Holcombe Blvd,Box 189 Houston TX USA 77030 7030 USA
Citazione:
H.F. Zhang et al., "gamma-radiation-induced single cell DNA damage as a measure of susceptibility to lung cancer: A preliminary report", INT J ONCOL, 17(2), 2000, pp. 399-404

Abstract

The comet assay is a sensitive and rapid method for detecting DNA single-strand and double-strand breaks and the individual cell's DNA repair profile. This pilot study was designed to determine whether the comet assay could measure inherited susceptibility to lung cancer. We applied the comet assayin the alkaline condition to test the DNA damage in gamma-irradiated and untreated cultured peripheral blood lymphocytes of 31 cases with previously untreated lung cancer and 39 controls. For each culture, 200 consecutive cells were examined and the number of cells with DNA uncoiling under electrophoresis ('comet cells') was recorded. The comet tail length (the radius of the nucleus plus the length of the migrated DNA) at 400-fold magnification was measured for the first 50 identified comet cells. The mean number of induced comet cells was significantly higher in cases (96.0 +/- 15.7) than matched controls (68.9 +/- 35.8) (P<0.05). However, no significant differencewas observed in induced comet tail length between cases and controls. Whenwe categorized the number of comet cells by the 75th percentile value in the controls, a higher number of comet cells was associated with significantly increased risk for lung cancer [odds ratio = 4.8 (confidence intervals of 1.5, 15.2)] after adjustment for age, sex, ethnicity and smoking status. The number of gamma-irradiation-induced comet cells (r=0.499, P<0.05) and comet tail length (r=0.520, P<0.05) correlated with the results on a previously reported Lung cancer susceptibility marker, bleomycin sensitivity. Also, the number of gamma-irradiation-induced comet cells correlated with the results of the benzo[a]pyrene diol epoxide mutagen sensitivity assay, which quantifies induced chromatid breaks (r=0.275, P<0.05). The comet assay might be a simple and inexpensive tool for detecting generic susceptibility to lung cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:16:31