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Titolo:
Sodium phosphate enhances plasmid DNA expression in vivo
Autore:
Hartikka, J; Bozoukova, V; Jones, D; Mahajan, R; Wloch, MK; Sawdey, M; Buchner, C; Sukhu, L; Barnhart, KM; Abai, AM; Meek, J; Shen, N; Manthorpe, M;
Indirizzi:
Vical Inc, Dept Cell Biol, San Diego, CA 92121 USA Vical Inc San Diego CAUSA 92121 Dept Cell Biol, San Diego, CA 92121 USA Vical Inc, Dept Mol Biol, San Diego, CA 92121 USA Vical Inc San Diego CA USA 92121 , Dept Mol Biol, San Diego, CA 92121 USA Vical Inc, Dept Pharmaceut Dev, San Diego, CA 92121 USA Vical Inc San Diego CA USA 92121 Pharmaceut Dev, San Diego, CA 92121 USA
Titolo Testata:
GENE THERAPY
fascicolo: 14, volume: 7, anno: 2000,
pagine: 1171 - 1182
SICI:
0969-7128(200007)7:14<1171:SPEPDE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRAMUSCULAR GENE-TRANSFER; MOUSE SKELETAL-MUSCLE; NAKED DNA; IN-VIVO; INORGANIC-PHOSPHATE; SUPERCOILED DNA; INJECTION; THERAPY; DELIVERY; MICE;
Keywords:
gene therapy; vectors; nonviral; expression; sodium phosphate; vehicle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Hartikka, J Vical Inc, Dept Cell Biol, 9373 Towne Ctr Dr, San Diego, CA 92121 USA Vical Inc 9373 Towne Ctr Dr San Diego CA USA 92121 A 92121 USA
Citazione:
J. Hartikka et al., "Sodium phosphate enhances plasmid DNA expression in vivo", GENE THER, 7(14), 2000, pp. 1171-1182

Abstract

Intramuscular injection of plasmid DNA results in myofiber cell expressionof proteins encoded by the DNA. The preferred vehicle for plasmid DNA injections has been saline (154 mM sodium chloride) or PBS (154 mM NaCl plus 10mM sodium phosphate). Here, it is shown that injection of luciferase or p-galactosidase encoding plasmid DNA in a 150 mM sodium phosphate vehicle into murine muscle resulted in a two- to seven-fold increase in transgene expression compared with DNA injected in saline or PBS. When the DNA encoded secreted alkaline phosphatase, preproinsulin or interferon, sodium phosphate vehicle increased their serum levels by two- to four-fold. When the DNA encoded mouse erythropoietin, sodium phosphate vehicle increased hematocrits by two-fold compared with DNA injected in saline. When the DNA encoded influenza nucleoprotein, sodium phosphate increased anti-nucleoprotein antibody titers by two-fold. The expression of luciferase from plasmid DNA instilledinto lung was increased five-fold compared with that in vehicle without sodium phosphate. Incubation of plasmid DNA with muscle extract or serum showed that sodium phosphate protected the DNA from degradation. Thus, a changefrom sodium chloride to sodium phosphate vehicle can enhance the expression of plasmid DNA in a tissue, possibly by inhibiting DNA degradation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 03:36:49