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Titolo:
Specific antibody promotes opsonization and PMN-mediated killing of phagocytosis-resistant Enterococcus faecium
Autore:
Rakita, RM; Quan, VC; Jacques-Palaz, K; Singh, KV; Arduino, RC; Mee, M; Murray, BE;
Indirizzi:
Virginia Mason Med Ctr, Seattle, WA 98111 USA Virginia Mason Med Ctr Seattle WA USA 98111 ed Ctr, Seattle, WA 98111 USA Univ Texas, Sch Med, Dept Internal Med, Ctr Study Emerging & Reemerging Pathogens, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 emerging Pathogens, Houston, TX 77030 USA Univ Texas, Sch Med, Div Infect Dis, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 ed, Div Infect Dis, Houston, TX 77030 USA Univ Texas, Sch Med, Dept Microbiol & Mol Genet, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 robiol & Mol Genet, Houston, TX 77030 USA
Titolo Testata:
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
fascicolo: 4, volume: 28, anno: 2000,
pagine: 291 - 299
SICI:
0928-8244(200008)28:4<291:SAPOAP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALTERNATIVE COMPLEMENT PATHWAY; GROUP-B STREPTOCOCCUS; MONOCLONAL-ANTIBODIES; SIALIC-ACID; ACTIVATION; NEUTROPHILS; OPSONOPHAGOCYTOSIS; IMMUNOGLOBULIN; ADHERENCE; SYSTEM;
Keywords:
Enterococcus faecium; phagocytosis; neutrophil; complement; antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Rakita, RM Virginia Mason Med Ctr, 1100 9th Ave,POB 900 Mail Stop C7-PUL, Seattle, WA98111 USA Virginia Mason Med Ctr 1100 9th Ave,POB 900 Mail Stop C7-PUL Seattle WA USA 98111
Citazione:
R.M. Rakita et al., "Specific antibody promotes opsonization and PMN-mediated killing of phagocytosis-resistant Enterococcus faecium", FEMS IM MED, 28(4), 2000, pp. 291-299

Abstract

Many clinical isolates of Enterococcus faecium are resistant to neutrophil(PMN)-mediated phagocytosis and killing in the presence of normal human serum. We have now examined the ability of specific polyclonal rabbit antibodies to promote opsonization and killing of phagocytosis-resistant E. faecium. Immune rabbit serum generated against formalin-killed E. faecium TX0016,: a phagocytosis-resistant strain, markedly promoted binding of TX0016 organisms to PMNs and PMN-mediated killing. These effects were dramatically reduced by (a) adsorption of immune serum with E. faecium TX0016, but not by adsorption with a strain of E. faecium susceptible to phagocytosis, and (b) incubation of immune serum with carbohydrate purified from TX0016, but not by incubation with a surface protein extract from TX0016. IgG purified fromimmune serum was unable by itself to promote bacterial binding to PMNs. However, specific IgG was able to promote binding to PMNs and PMN-mediated killing in the presence of normal human serum as a complement source, as wereF(ab')(2) and Fab fragments produced from it, and the alternative pathway of complement was sufficient to promote IgG- and F(ab')(2)-mediated opsonization. PMN complement receptor type 3, but not complement receptor type 1, was involved in bacterial binding to PMNs induced by the combination of F(ab')(2) fragments and normal human serum. These results suggest that opsonization by antibodies potentially directed against bacterial carbohydrate, inconjunction with complement activation, has an important role in the host defense against phagocytosis-resistant E. faecium. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 05/08/20 alle ore 09:39:20