Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Synaptic plasticity in the basolateral amygdala in transgenic mice expressing dominant-negative cAMP response element-binding protein (CREB) in forebrain
Autore:
Rammes, G; Steckler, T; Kresse, A; Schutz, G; Zieglgansberger, W; Lutz, B;
Indirizzi:
Max Planck Inst Psychiat, D-80804 Munich, Germany Max Planck Inst Psychiat Munich Germany D-80804 D-80804 Munich, Germany German Canc Res Ctr, Dept Mol Biol Cell 1, D-69120 Heidelberg, Germany German Canc Res Ctr Heidelberg Germany D-69120 69120 Heidelberg, Germany
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 12, anno: 2000,
pagine: 2534 - 2546
SICI:
0953-816X(200007)12:7<2534:SPITBA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; GENE-TRANSCRIPTION; LATERAL AMYGDALA; CA2+ CHANNELS; IN-VITRO; FEAR; MEMORY; RAT; INDUCTION; NMDA;
Keywords:
amygdala; long-term depression; long-term potentiation; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Rammes, G Max Planck Inst Psychiat, Kraepelinstr 2-10, D-80804 Munich, Germany Max Planck Inst Psychiat Kraepelinstr 2-10 Munich Germany D-80804
Citazione:
G. Rammes et al., "Synaptic plasticity in the basolateral amygdala in transgenic mice expressing dominant-negative cAMP response element-binding protein (CREB) in forebrain", EUR J NEURO, 12(7), 2000, pp. 2534-2546

Abstract

Electrophysiological and behavioural experiments were performed in transgenic mice expressing a dominant-negative form of cAMP response element-binding protein (CREBA133) in the limbic system. In control littermate in vitro slice preparation, tetanizing the lateral amygdala-basolateral amygdala (BLA) pathway with a single train (100 Hz for 1 s) produced short-term potentiation (STP) in the BLA. Five trains (10-s interstimulus interval) induced long-term potentiation (LTP), which was completely blocked by the N-methyl-d-aspartate (NMDA) receptor antagonist d(-)-2-amino-5-phosphonopentanoic acid (AP5; 50 mu m). When GABAergic (gamma-aminobutyric acid) inhibition was blocked by picrotoxin (10 mu m), LTP became more pronounced. Low-frequency stimulation (1 Hz for 15 min) induced either long-term depression (LTD) or depotentiation. LTD remained unaffected by AP5 (50 mu m) or by the L- and T-type Ca2+-channel blockers nifedipine (20 mu m) and Ni2+ (50 mu m), but wasprevented by picrotoxin (10 mu m), indicating a GABAergic link in the expression of LTD in the BLA. When conditioned fear was tested, a mild impairment was seen in one of three transgenic lines only. Although high levels of mRNA encoding CREBA133 lead to downregulation of endogenous CREB, expression of LTP and depotentiation were unaltered in BLA of these transgenic animals. These results could suggest that residual CREB activity was still present or that CREB per se is dispensable. Alternatively, other CREB-like proteins were able to compensate for impaired CREB function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:40:34