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Titolo:
Immature dendritic cells generated with low doses of GM-CSF in the absenceof IL-4 are maturation resistant and prolong allograft survival in vivo
Autore:
Lutz, MB; Suri, RM; Niimi, M; Ogilvie, ALJ; Kukutsch, NA; Rossner, S; Schuler, G; Austyn, JM;
Indirizzi:
Univ Erlangen, Dept Dermatol, D-91052 Erlangen, Germany Univ Erlangen Erlangen Germany D-91052 rmatol, D-91052 Erlangen, Germany Univ Oxford, John Radcliffe Hosp, Nuffield Dept Surg, Oxford OX3 9DU, England Univ Oxford Oxford England OX3 9DU ld Dept Surg, Oxford OX3 9DU, England
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 30, anno: 2000,
pagine: 1813 - 1822
SICI:
0014-2980(200007)30:7<1813:IDCGWL>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE BONE-MARROW; ANTIGEN-PRESENTING FUNCTION; EPIDERMAL LANGERHANS CELLS; COLONY-STIMULATING FACTOR; CLASS-II MOLECULES; IN-VIVO; POSITIVE SELECTION; INVARIANT CHAIN; LARGE NUMBERS; B-CELLS;
Keywords:
dendritic cell; T cell; tolerance; transplantation; unresponsiveness;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Lutz, MB Univ Erlangen, Dept Dermatol, Hartmannstr 14, D-91052 Erlangen, Germany Univ Erlangen Hartmannstr 14 Erlangen Germany D-91052 n, Germany
Citazione:
M.B. Lutz et al., "Immature dendritic cells generated with low doses of GM-CSF in the absenceof IL-4 are maturation resistant and prolong allograft survival in vivo", EUR J IMMUN, 30(7), 2000, pp. 1813-1822

Abstract

Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitorsin low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in high concentrations of GM-CSF (GM(hl) DC) or in low GM-CSF plus IL-4 (GM(lo)/IL-4 DC). An effect of IL-4 on maturation was observed only at low but not high doses of GM-CSF, Compared to mature DC, GM(lo) DC were phenotypically immature, weak stimulators of allogeneic and peptide-specific T cell responses, but substantially more potent in presentation of native protein. Immature GM(lo) DC were resistant to maturation by lipopolysaccharide, TNF-cr or anti-CD40 monoclonal antibodies, as the expression of co-stimulatory molecules was not increased, and stimulatory activity in oxidative mitogenesis was not enhanced, These maturation-resistant immatureGM(lo) DC induced T cell unresponsiveness in vitro and in vivo. GM(lo) DC also prolonged haplotype-specific cardiac allograft survival (from 8 days to >100 days median survival time) when they were administered 7 days (but not 3, 14 or 28 days) before transplantation. Our findings may have important implications for future studies in T cell tolerance induction in vivo.

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Documento generato il 18/09/20 alle ore 17:19:19