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Titolo:
Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteoglycans PG-M/versican and aggrecan
Autore:
Perissinotto, D; Iacopetti, P; Bellina, I; Doliana, R; Colombatti, A; Pettway, Z; Bronner-Fraser, M; Shinomura, T; Kimata, K; Morgelin, M; Lofberg, J; Perris, R;
Indirizzi:
IRCCS, Natl Canc Inst, CRO, Div Expt Oncol 2, I-33081 Aviano, PN, Italy IRCCS Aviano PN Italy I-33081 Div Expt Oncol 2, I-33081 Aviano, PN, Italy Univ Udine, Dept Med Sci & Technol, I-35100 Padua, Italy Univ Udine Padua Italy I-35100 t Med Sci & Technol, I-35100 Padua, Italy CALTECH, Div Biol, Pasadena, CA USA CALTECH Pasadena CA USACALTECH, Div Biol, Pasadena, CA USA Aichi Med Univ, Inst Mol Sci Med, Nagakute, Aichi 48011, Japan Aichi Med Univ Nagakute Aichi Japan 48011 d, Nagakute, Aichi 48011, Japan Univ Lund, Dept Cell & Mol Biol, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100 Dept Cell & Mol Biol, S-22100 Lund, Sweden Univ Uppsala, Dept Environm & Dev Biol, S-75236 Uppsala, Sweden Univ Uppsala Uppsala Sweden S-75236 & Dev Biol, S-75236 Uppsala, Sweden
Titolo Testata:
DEVELOPMENT
fascicolo: 13, volume: 127, anno: 2000,
pagine: 2823 - 2842
SICI:
0950-1991(200007)127:13<2823:ANCCMI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN SULFATE PROTEOGLYCAN; EXTRACELLULAR-MATRIX; CHICK-EMBRYO; ELECTRON-MICROSCOPY; AXOLOTL EMBRYOS; LECTIN DOMAINS; VERSICAN; EXPRESSION; MOUSE; NOTOCHORD;
Keywords:
PG-M/versican; neural crest; cell migration; aggrecan; proteoglycan; chick;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Perris, R IRCCS, Natl Canc Inst, CRO, Div Expt Oncol 2, Via Pedemontana Occidentale 12, I-33081 Aviano, PN, Italy IRCCS Via Pedemontana Occidentale 12 Aviano PN Italy I-33081 aly
Citazione:
D. Perissinotto et al., "Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteoglycans PG-M/versican and aggrecan", DEVELOPMENT, 127(13), 2000, pp. 2823-2842

Abstract

It has been proposed that hyaluronan-binding proteoglycans play an important role as guiding cues during neural crest (NC) cell migration, but their precise function has not been elucidated. In this study, we examine the distribution, structure and putative role of the two major hyaluronan-binding proteoglycans, PG-M/versicans and aggrecan, during the course of avian NC development. PG-M/versicans V0 and V1 are shown to be the prevalent isoformsat initial and advanced phases of NC cell movement, whereas the V2 and V3 transcripts are first detected following gangliogenesis. During NC cell dispersion, mRNAs for PG-M/versicans V0/V1 are transcribed by tissues lining the NC migratory pathways, as well as by tissues delimiting nonpermissive areas. Immunohistochemistry confirm the deposition of the macromolecules in these regions and highlight regional differences in the density of these proteoglycans. PGM/versicans assembled within the sclerotome rearrange from aninitially uniform distribution to a preferentially caudal localization, both at the mRNA and protein level. This reorganization is a direct consequence of the metameric NC cell migration through the rostral portion of the somites. hs suggested by previous in situ hybridizations, aggrecan shows a virtually opposite distribution to PG-M/versicans being confined to the perinotochordal ECM and extending dorsolaterally in a segmentally organized manner eventually to the entire spinal cord at axial levels interspacing the ganglia. PG-M/versicans purified from the NC migratory routes are highly polydispersed, have an apparent M-r of 1,200-2,000 kDa, are primarily substituted with chondroitin-6-sulfates and, upon chondroitinase ABC digestion, are found to be composed of core proteins with apparent Mi of 360-530,000. TEM/rotary shadowing analysis of the isolated PG-M/versicans confirmed that they exhibit the characteristic bi-globular shape, have core proteins with sizes predicted for the V0/V1 isoforms and carry relatively few extended glycosaminoglycan chains. Orthotopical implantation of PG-M/versicans immobilized onto transplantable micromembranes tend to 'attract' moving cells toward them, whereas similar implantations of a notochordal type-aggrecan retain both single and cohorts of moving NC cells in close proximity of the implantand thereby perturb their spatiotemporal migratory pattern. NC cells fail to migrate through three-dimensional collagen type I-aggrecan substrata in vitro, but locomote in a haptotactic manner through collagen type I-PG-M/versican V0 substrata via engagement of HNK-1 antigen-bearing cell surface components. The present data suggest that PG-M/versicans and notochordal aggrecan exert divergent guiding functions during NC cell dispersion, which aremediated by both their core proteins and glycosaminoglycan side chains andmay involve 'haptotactic-like' motility phenomena. Whereas aggrecan defines strictly impenetrable embryonic areas, PG-M/versicans are central components of the NC migratory pathways favoring the directed movement of the cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 11:43:40