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Titolo:
Cloning and functional characterization of a novel c-mpl variant expressedin human CD34 cells and platelets
Autore:
Li, JZ; Sabath, DF; Kuter, DJ;
Indirizzi:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Hematol Oncol Unit, Boston,MA 02114 USA Harvard Univ Boston MA USA 02114 Hematol Oncol Unit, Boston,MA 02114 USA Univ Washington, Dept Med, Seattle, WA USA Univ Washington Seattle WA USA niv Washington, Dept Med, Seattle, WA USA
Titolo Testata:
CYTOKINE
fascicolo: 7, volume: 12, anno: 2000,
pagine: 835 - 844
SICI:
1043-4666(200007)12:7<835:CAFCOA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD-PLATELETS; IMMORTALIZES HEMATOPOIETIC PROGENITORS; PROTEIN-TYROSINE PHOSPHORYLATION; TRUNCATED CYTOKINE RECEPTOR; THROMBOPOIETIN RECEPTOR; MEGAKARYOCYTE GROWTH; V-MPL; LIGAND; MICE; PROLIFERATION;
Keywords:
CD34 cells; c-mpl; megakaryocyte; platelet; thrombopoietin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kuter, DJ Harvard Univ, Massachusetts Gen Hosp, Sch Med, Hematol Oncol Unit, 100 Blossom St, Boston, MA 02114 USA Harvard Univ 100 Blossom St Boston MA USA 02114 on, MA 02114 USA
Citazione:
J.Z. Li et al., "Cloning and functional characterization of a novel c-mpl variant expressedin human CD34 cells and platelets", CYTOKINE, 12(7), 2000, pp. 835-844

Abstract

The thrombopoietin receptor, c-mpl, is a crucial element not only in thrombopoietin (TPO)-initiated signaling pathways but also in the regulation of the circulating amount of TPO, We have identified a new c-mpl isoform, called c-mpl-del, that lacks 72 bp (24 amino acids) in the extracellular regionof c-mpl and arises as a consequence of alternative RNA splicing between exons 8 and 9, c-mpl-del is expressed along with c-mpl-wt in blood mononuclear cells, CD34(+) cells, megakaryocytes, and platelets prepared from eithernormal donors or ET patients, although its relative expression appears to increase with megakaryocyte differentiation. The c-mpl-del-transfected cells expressed greater amounts of c-mpl-del RNA and protein than the comparable c-mpl-wt-transfected cells, however flow cytometry analysis could not detect any c-mpl receptor on the surface of the c-mpl-del-transfected cells. Further evidence for the absence of surface c-mpl-del was that in contrast to cells transfected with c-mpl-wt, those transfected with c-mpl-del did notgrow in response to TPO, failed to undergo tyrosine phosphorylation of TPO-specific signal molecules, and did not bind I-125-rHuTPO. Taken together, these results demonstrate that c-mpl-del, a naturally occurring variant of c-mpl, fails to be incorporated into the cell membrane but might serve as amechanism to decrease the overall expression of functional c-mpl late in megakaryocyte differentiation. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:51:18