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Titolo:
Gastrointestinal stability and absorption of insulin in suckling pigs
Autore:
Shen, WH; Xu, RJ;
Indirizzi:
Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China Univ HongKong Hong Kong Hong Kong Peoples R China Kong, Peoples R China
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 3, volume: 125, anno: 2000,
pagine: 389 - 401
SICI:
1095-6433(200003)125:3<389:GSAAOI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; NEONATAL PIGS; INTESTINAL-MUCOSA; MILK; MACROMOLECULES; TRANSMISSION; PIGLET; RAT; IDENTIFICATION; ENTEROCYTES;
Keywords:
fluorescein-isothiocyanate labeled insulin (FITC-insulin); intestinal absorption; suckling pigs; milk-borne insulin; stability; gastrointestinal (GI) tract; size-exclusion chromatography; radioimmunoassay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Shen, WH Indiana Univ, Sch Med, Dept Pediat, Wells Ctr Pediat Res,Riley Hosp Children, 702 Barnhill Dr,Room 2640, Indianapolis, IN 46202 USA Indiana Univ 702 Barnhill Dr,Room 2640 Indianapolis IN USA 46202
Citazione:
W.H. Shen e R.J. Xu, "Gastrointestinal stability and absorption of insulin in suckling pigs", COMP BIOC A, 125(3), 2000, pp. 389-401

Abstract

Stability and absorption of orally administered fluorescein-isothiocyanatelabeled insulin (FITC-insulin) in the gastrointestinal (GI) tract were investigated in newborn and 3-day-old pigs. The uptake of FITC-insulin by the intestinal epithelial cells was visualized using confocal laser scanning microscopy. Following oral administration, 3 h later 56 and 88% of orally administered fluorescence was found in the GI tract in newborn and 3-day-old piglets, respectively. Chromatographic analysis revealed that 15-37% of fluorescence recovered from the gastric and proximal intestinal contents was eluted in the void volume of a Sephadex G-25 column. It was also observed that oral administration of FITC-insulin at a dose of 100 nmol/kg body weight led to a significant decrease in blood glucose in newborn pigs (P < 0.05) but not in 3-day-old pigs. Microscopic examination showed that FITC-insulin was taken up via the vesicular transport mechanism throughout the whole small intestine but the ileum appeared to be a preferred site for FITC-insulintransport in newborn pigs. In 3-day-old pigs, the uptake of FITC-insulin occurred only in the distal part of the small intestine. These findings suggest that milk-borne insulin may partially survive in the GI lumen and subsequently act on the gastrointestinal tract in suckling piglets, while GI absorption of milk-borne insulin is limited to newborn pigs. (C) 2000 ElsevierScience Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 21:46:18