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Titolo:
On accuracy and precision of a HPLC method for measurement of urine porphyrin concentrations
Autore:
Zuijderhoudt, FMJ; Koehorst, SGA; Kluitenberg, WE; Dorresteijn-de Bok, J;
Indirizzi:
Deventer Ziekenhuis, Dept Clin Chem, NL-7400 GC Deventer, Netherlands Deventer Ziekenhuis Deventer Netherlands NL-7400 GC eventer, Netherlands
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 3, volume: 38, anno: 2000,
pagine: 227 - 230
SICI:
1434-6621(200003)38:3<227:OAAPOA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; EXCRETION; PROFILES;
Keywords:
calibration of standards; recovery of porphyrins; influence of type of porphyria on test results;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Zuijderhoudt, FMJ Deventer Ziekenhuis, Dept Clin Chem, Postbus 5001, NL-7400 GC Deventer, Netherlands Deventer Ziekenhuis Postbus 5001 Deventer Netherlands NL-7400 GC
Citazione:
F.M.J. Zuijderhoudt et al., "On accuracy and precision of a HPLC method for measurement of urine porphyrin concentrations", CLIN CH L M, 38(3), 2000, pp. 227-230

Abstract

We studied the accuracy and precision of a HPLC method for determination of porphyrins in urine. A commercial standard solution appeared to contain less porphyrins than indicated by the manufacturer, since calibration resulted in lower concentrations of uroporphyrin and coproporphyrin: 16% and 8%, respectively. Coefficients of variation for the measurement of uro-, hepta-, copro I and copro III porphyrins in samples of patients with and without porphyria were often much less than 15%. Comparison of measurements with and without calibrated standards revealed differences for uroporphyrin and coproporphyrin of 27% and 5%, respectively. Recovery of added uroporphyrin and coproporphyrin was 99%. The main cause of the variability in test resultswas apparently the improperly calibrated standard solutions. The precisionof porphyrin measurements was not influenced by the type of porphyria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:00:55