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Titolo:
Male gender predisposes to development of endotoxic shock in the rat
Autore:
Losonczy, G; Kriston, T; Szabo, A; Muller, V; Harvey, J; Hamar, P; Heemann, U; Baylis, C;
Indirizzi:
W Virginia Univ, Dept Physiol, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 Physiol, Morgantown, WV 26506 USA Semmelweis Univ Med, Dept Pulmonol & Pathophysiol, H-1125 Budapest, Hungary Semmelweis Univ Med Budapest Hungary H-1125 ol, H-1125 Budapest, Hungary Univ Klinikum Essen, Nephrol Abt, D-45122 Essen, Germany Univ Klinikum Essen Essen Germany D-45122 ol Abt, D-45122 Essen, Germany
Titolo Testata:
CARDIOVASCULAR RESEARCH
fascicolo: 1, volume: 47, anno: 2000,
pagine: 183 - 191
SICI:
0008-6363(200007)47:1<183:MGPTDO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; GENE-EXPRESSION; IMMUNE-RESPONSE; SEX-HORMONES; INDUCTION; LIVER; LIPOPOLYSACCHARIDE; AORTA; MACROPHAGES; MECHANISMS;
Keywords:
endotoxins; gender; nitric oxide; shock;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Losonczy, G W Virginia Univ, Dept Physiol, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 gantown, WV 26506 USA
Citazione:
G. Losonczy et al., "Male gender predisposes to development of endotoxic shock in the rat", CARDIO RES, 47(1), 2000, pp. 183-191

Abstract

Objective: After intravenous (i.v.) injection of lipopolysaccharide (LPS) macrophages release nitric oxide (NO) due to the expression of the inducible NO synthase (iNOS). After LPS NO is abundantly produced also in the cardiovascular system and may contribute to the development of hypotension and shock. Since the immune response, the synthesis of NO and the regulation of blood pressure (BP) differ between males and females, in the present study the effect of LPS on BP, renal function, the plasma and urinary concentration of the metabolites of NO as well as the splenic and aortic expression ofthe iNOS gene were compared between male and female rats. Methods: BP and renal function were measured in anesthetized rats following the i.v. injection of LPS (E. coli, 4 mg/kg). The NOx and NOx (metabolites of NO=NOx) concentration was measured by the Griess reaction. The iNOS gene expression wasstudied by RT-PCR. Results: Four hours after LPS, BP of males (n=9) was reduced by 63+/-12 mmHg versus 10+/-4 in females (n=7, P<0.005). Aminoguanidine, a selective inhibitor of iNOS, prevented the reduction of BP in males. The plasma concentration of NOx (P-NOx, mu M) was lower in hypotensive males (128+/-20) than in normotensive females (235+/-29, P<0.005). Males also exhibited lower urinary NO, excretion (UNOxV) after LPS (P<0.001 vs, females). Prior castration of males provided protection against hypotension (fall of BP: -4+/-4 mmHg, n=6, P<0.02 versus males) and resulted in higher P-NOx as well as UNOxV (both P<0.001 versus males and not different from females). Prior ovariectomy (n=5) had no influence on the hemodynamic and NOx response to LPS. Male rats displayed enhanced aortic iNOS/beta-actin ratio relative to females after LPS (n=3 in each group, P<0.05). Conclusions: (1) Malegender may sensitize to LPS-induced shock and (2) sensitivity of males to endotoxin is associated with an attenuated, not exaggerated total rate of NO synthesis. (C) 2000 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:28:20