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Titolo:
Gene transfer into rat renal cells using adeno-associated virus vectors
Autore:
Shimpo, M; Ikeda, U; Maeda, Y; Ueno, S; Ikeda, M; Minota, S; Takizawa, T; Urabe, M; Kume, A; Monahan, J; Ozawa, K; Shimada, K;
Indirizzi:
Jichi Med Sch, Ctr Mol Med, Div Genet Therapeut, Minamimaki, Tochigi 3290498, Japan Jichi Med Sch Minamimaki Tochigi Japan 3290498 ki, Tochigi 3290498, Japan Jichi Med Sch, Ctr Mol Med, Dept Cardiol, Minamimaki, Tochigi 3290498, Japan Jichi Med Sch Minamimaki Tochigi Japan 3290498 ki, Tochigi 3290498, Japan Jichi Med Sch, Dept Clin Immunol, Minamimaki, Tochigi 3290498, Japan JichiMed Sch Minamimaki Tochigi Japan 3290498 ki, Tochigi 3290498, Japan Jichi Med Sch, Dept Anat, Minamimaki, Tochigi 3290498, Japan Jichi Med Sch Minamimaki Tochigi Japan 3290498 ki, Tochigi 3290498, Japan Avigen Inc, Alameda, CA USA Avigen Inc Alameda CA USAAvigen Inc, Alameda, CA USA
Titolo Testata:
AMERICAN JOURNAL OF NEPHROLOGY
fascicolo: 3, volume: 20, anno: 2000,
pagine: 242 - 247
SICI:
0250-8095(200005/06)20:3<242:GTIRRC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOASSOCIATED VIRUS; IN-VIVO; NONDIVIDING CELLS; MAMMALIAN KIDNEY; MESANGIAL CELLS; AAV VECTORS; FACTOR-IX; EXPRESSION; RETROVIRUS; THERAPY;
Keywords:
AAV vector; gene transfer; mesangial cell; renal tubule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ozawa, K Jichi Med Sch, Ctr Mol Med, Div Genet Therapeut, Minamimaki, Tochigi 3290498, Japan Jichi Med Sch Minamimaki Tochigi Japan 3290498 gi 3290498, Japan
Citazione:
M. Shimpo et al., "Gene transfer into rat renal cells using adeno-associated virus vectors", AM J NEPHR, 20(3), 2000, pp. 242-247

Abstract

Adeno-associated virus (AAV) vectors have a number of attractive features,including lack of cytotoxicity, ability to transduce nondividing cells, and long-term transgene expression. We investigated whether rat renal cells could be efficiently transduced with AAV vectors. Rat glomerular mesangial cells were transduced with AAV-lacZ vector containing beta-galactosidase gene in vitro, and the expression of beta-galactosidase was evaluated by X-galstaining and ELISA, For ex vivo experiments, sections of rat kidneys were incubated with AAV-lacZ, and then evaluated by X-gal histochemical staining, The level of beta-galactosidase expression in cultured rat mesangial cells increased in a dose-dependent manner (ranging from 1 x 10(5) to 5 x 10(6)particles/cell). When transduced with 5 x 10(6) vector particles/cell of AAV-lacZ, about 50% of mesangial cells were stained positively with X-gal, and the level of beta-galactosidase expression reached 9.9 +/- 1.5 ng/mg protein. Expression was detectable during the culture period for at least 7 days. X-gal histochemical examination of the ex vivo transduced renal tissue revealed tubular cell and interstitial tissue staining. However, gene transfer was not clearly observed in glomeruli. These findings suggest that AAV vectors have the potential for gene therapy of renal diseases. Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:15:03