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Titolo:
Specific detection and quantitation of SCC antigen 1 and SCC antigen 2 mRNAs by fluorescence-based asymmetric semi-nested reverse transcription PCR
Autore:
Murakami, A; Suminami, Y; Sakaguchi, Y; Nawata, S; Numa, F; Kishi, F; Kato, H;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Obstet & Gynecol, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Ctr Gene Res, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan
Titolo Testata:
TUMOR BIOLOGY
fascicolo: 4, volume: 21, anno: 2000,
pagine: 224 - 234
SICI:
1010-4283(200007/08)21:4<224:SDAQOS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; SERINE-PROTEASE INHIBITOR; TUMOR-ANTIGEN; CYSTEINE PROTEINASES; UTERINE CERVIX; ELECTROPHORESIS; SERPIN; TA-4; EXPRESSION; LOCUS;
Keywords:
SCCA; SCCA1mRNA; SCCA2 mRNA; amplisensor assay system; amplisensor primer; asymmetric semi-nested RT-PCR; quantitation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kato, H Yamaguchi Univ, Sch Med, Dept Obstet & Gynecol, 1-1-1 Minamikogushi, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ 1-1-1 Minamikogushi Ube Yamaguchi Japan 7558505 pan
Citazione:
A. Murakami et al., "Specific detection and quantitation of SCC antigen 1 and SCC antigen 2 mRNAs by fluorescence-based asymmetric semi-nested reverse transcription PCR", TUMOR BIOL, 21(4), 2000, pp. 224-234

Abstract

Squamous cell carcinoma antigen (SCCA) is expressed in normal squamous epithelia and malignant squamous cell tissues. The serum level of SCCA has been used to evaluate treatment efficacy, clinical course of disease, and recurrence. SCCA is produced by at least two genes (SCCA I and SCCA2); both of them have been located on chromosome 18q21.3. It has been difficult to examine the expression levels of SCCA1 and SCCA2 mRNAs separately because of their high homology at nucleotide level. In the present study, asymmetric semi-nested reverse transcription PCR, based on the principle of fluorescence energy transfer, enabled to quantitate the copy numbers of both SCCA1 and SCCA2 mRNAs. Using this method, the expression levels of these mRNAs were evaluated in normal and malignant squamous tissues. The copy number of SCCA2 mRNA was higher in malignant tissues than in normal tissues, while those ofSCCA1 mRNA did not significantly differ between normal and malignant tissues. These data indicate that specific quantitation of the expression level of SCCA2 mRNA may be useful for the diagnosis and management of patients with squamous cell carcinoma. Copyright (C) 2000 S. Karger AG. Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:47:34