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Titolo:
Identification and characterization of a newly recognized population of high-Na+, low-K+ low-density sickle and normal red cells
Autore:
Bookchin, RM; Etzion, Z; Sorette, M; Mohandas, N; Skepper, JN; Lew, VL;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ley Lab, Berkeley, CA 94720 USA Univ Cambridge, Sch Biol Sci, MultiImaging Ctr, Cambridge CB2 3DY, EnglandUniv Cambridge Cambridge England CB2 3DY Ctr, Cambridge CB2 3DY, England Univ Cambridge, Physiol Lab, Cambridge CB2 3EG, England Univ Cambridge Cambridge England CB2 3EG Lab, Cambridge CB2 3EG, England
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 14, volume: 97, anno: 2000,
pagine: 8045 - 8050
SICI:
0027-8424(20000705)97:14<8045:IACOAN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOGLOBIN CONCENTRATION; LIGHT-SCATTERING; VOLUME; RETICULOCYTES; ACCURATE; BLOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Bookchin, RM Yeshiva Univ Albert Einstein Coll Med, Dept Med, 1300 Morris Pk Ave,Rm 913U, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave,Rm 913U Bronx NY USA 10461
Citazione:
R.M. Bookchin et al., "Identification and characterization of a newly recognized population of high-Na+, low-K+ low-density sickle and normal red cells", P NAS US, 97(14), 2000, pp. 8045-8050

Abstract

We describe a population of sickle cell anemia red cells (SS RBCs) (approximate to 4%) and a smaller fraction of normal RBCs (<0.03%) that fail to dehydrate when permeabilized to K+ with either valinomycin or elevated internal Ca2+. The nonshrinking, valinomycin-resistant (val-res) fractions, firstdetected by flow cytometry of density-fractionated SS RBCs, constituted upto 60% of the lightest, reticulocyte-rich (R1) cell fraction, and progressively smaller portions of the slightly denser R2 cells and discocytes. R1 val-res RBCs had a mean cell hemoglobin concentration of approximate to 21 gof Hb per dl, and many had an elongated shape like "irreversibly sickled cells," suggesting a dense SS cell origin. Of three possible explanations for val-res cells, failure of valinomycin to K+-permeabilize the cells, low co-ion permeability, or reduced driving K+ gradient, the latter proved responsible: Both SS and normal val-res RBCs were consistently high-Na+ and low-K+, even when processed entirely in Na-free media. Ca2+ + A23187-induced K+-permeabilization of SS R1 fractions revealed a similar fraction of cal-rescells, whose Rb-86 uptake showed both high Na/K pump and leak fluxes, val-res/calres RBCs might represent either a distinct erythroid genealogy, or an "end-stage" of normal and SS RBCs, This paper focuses on the discovery, basic characterization, and exclusion of artifactual origin of this RBC fraction. Many future studies will be needed to clarify their mechanism of generation and full pathophysiological significance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:57:51