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Titolo:
Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast
Autore:
Lyons, TJ; Gasch, AP; Gaither, LA; Botstein, D; Brown, PO; Eide, DJ;
Indirizzi:
Univ Missouri, Dept Nutr Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Nutr Sci, Columbia, MO 65211 USA Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Dept Biochem, Stanford, CA 94305 USA Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 d, Dept Genet, Stanford, CA 94305 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 14, volume: 97, anno: 2000,
pagine: 7957 - 7962
SICI:
0027-8424(20000705)97:14<7957:GCOTZZ>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; ALCOHOL-DEHYDROGENASE; GENE-EXPRESSION; ZYMOMONAS-MOBILIS; LACZ FUSIONS; CLONED GENES; ENCODES; PROTEIN; TRANSPORTER; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Eide, DJ Univ Missouri, Dept Nutr Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Sci, Columbia, MO 65211 USA
Citazione:
T.J. Lyons et al., "Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast", P NAS US, 97(14), 2000, pp. 7957-7962

Abstract

The Zap1p transcription factor senses cellular zinc status and increases expression of its target genes in response to zinc deficiency. Previously known Zap1p-regulated genes encode the Zrt1p, Zrt2p, and Zrt3p zinc transporter genes and Zap1p itself. To allow the characterization of additional genes in yeast important for zinc homeostasis, a systematic study of gene expression on the genome-wide scale was used to identify other Zap1p target genes. Using a combination of DNA microarrays and a computer-assisted analysis of shared motifs in the promoters of similarly regulated genes, we identified 46 genes that are potentially regulated by Zap1p. Zap1p-regulated expression of seven of these newly identified target genes was confirmed independently by using lacZ reporter fusions, suggesting that many of the remainingcandidate genes are also Zap1p targets. Our studies demonstrate the efficacy of this combined approach to define the regulon of a specific eukaryotictranscription factor.

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Documento generato il 06/04/20 alle ore 08:09:56