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Titolo:
Importance of sequences adjacent to the terminal tripeptide in the import of a peroxisomal Candida tropicalis protein in plant peroxisomes
Autore:
Bongcam, V; Petetot, JMC; Mittendorf, V; Robertson, EJ; Leech, RM; Qin, YM; Hiltunen, JK; Poirier, Y;
Indirizzi:
Univ Lausanne, Inst Ecol Biol & Physiol Vegetales, CH-1015 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1015 CH-1015 Lausanne, Switzerland Univ York, Dept Biol, York YO1 5DD, N Yorkshire, England Univ York York NYorkshire England YO1 5DD YO1 5DD, N Yorkshire, England Univ Oulu, Bioctr, FIN-90570 Oulu, Finland Univ Oulu Oulu Finland FIN-90570 v Oulu, Bioctr, FIN-90570 Oulu, Finland Univ Oulu, Dept Biochem, FIN-90570 Oulu, Finland Univ Oulu Oulu Finland FIN-90570 , Dept Biochem, FIN-90570 Oulu, Finland
Titolo Testata:
PLANTA
fascicolo: 1, volume: 211, anno: 2000,
pagine: 150 - 157
SICI:
0032-0935(200006)211:1<150:IOSATT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACYL-COA OXIDASE; TOBACCO LEAF PEROXISOMES; ACID BETA-OXIDATION; TARGETING SIGNAL; SACCHAROMYCES-CEREVISIAE; ISOCITRATE LYASE; RAT-LIVER; IN-VIVO; GLYOXYSOMAL MEMBRANES; TRIFUNCTIONAL ENZYME;
Keywords:
acyl-CoA oxidase; Arabidopsis; multifunctional protein; glyoxysome; peroxisome; peroxisome targeting signal;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Poirier, Y Univ Lausanne, Inst Ecol Biol & Physiol Vegetales, CH-1015 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1015 sanne, Switzerland
Citazione:
V. Bongcam et al., "Importance of sequences adjacent to the terminal tripeptide in the import of a peroxisomal Candida tropicalis protein in plant peroxisomes", PLANTA, 211(1), 2000, pp. 150-157

Abstract

The peroxisome targeting signal (PTS) required for import of the rat acyl-CoA oxidase (AOX; EC 1.3.3.6) and the Candida tropicalis multifunctional protein (MFP) in plane peroxisomes was assessed in transgenic Arabidopsis thaliana (L. ) Heynh. The native rat AOX accumulated in peroxisomes in A. thaliana cotyledons and targeting was dependent on the presence of the C-terminal tripeptide S-K-L. In contrast, the native C. tropicalis MFP, containing the consensus PTS sequence A-K-I was not targeted to plant peroxisomes. Modification of the carboxy terminus to the S-K-L tripeptide also failed to deliver the MFP to peroxisomes while addition of the last 34 amino acids of the Brassica napus isocitrate lyase, containing the terminal tripeptide S-R-M, enabled import of the fusion protein into peroxisomes. These results underline the influence of the amino acids adjacent to the terminal tripeptide of the C. tropicalis MFP on peroxisomal targeting, even in the context of aprotein having a consensus PTS sequence S-K-L.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:00:46