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Titolo:
Recognition of GT mismatches by Vsr mismatch endonuclease
Autore:
Fox, KR; Allinson, SL; Sahagun-Krause, H; Brown, T;
Indirizzi:
Univ Southampton, Sch Biol Sci, Dept Biochem & Mol Biol, Southampton SO16 7PX, Hants, England Univ Southampton Southampton Hants England SO16 7PX 6 7PX, Hants, England Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England Univ Southampton Southampton Hants England SO17 1BJ 7 1BJ, Hants, England
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 13, volume: 28, anno: 2000,
pagine: 2535 - 2540
SICI:
0305-1048(20000701)28:13<2535:ROGMBV>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI K-12; SHORT PATCH REPAIR; CYTOSINE METHYLATION; CRYSTAL-STRUCTURE; DNA DUPLEX; GENE; 5-METHYL-CYTOSINE; DIFLUOROTOLUENE; CLEAVAGE; ADENINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Fox, KR Univ Southampton, Sch Biol Sci, Dept Biochem & Mol Biol, Bassett Crescent E, Southampton SO16 7PX, Hants, England Univ Southampton Bassett Crescent E Southampton Hants England SO16 7PX
Citazione:
K.R. Fox et al., "Recognition of GT mismatches by Vsr mismatch endonuclease", NUCL ACID R, 28(13), 2000, pp. 2535-2540

Abstract

The Vsr mismatch endonuclease recognises the sequence CIWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5'-side of the mispaired thymine, By using base analogues of Gand T we have explored the functional groups on the mismatch pair which are recognised by the enzyme. Removal of the thymine 5-methyl group causes a 60% reduction in activity, while removing the 2-amino group of guanine reduces cleavage by 90%, Placing 2-aminopurine or nebularine opposite T generates mismatches which are cut at a much lower rate (0.1%), When either base is removed, generating a pseudoabasic site (1',2'-dideoxyribose), the enzymestill produces site-specific cleavage, but at only 1% of the original rate. Although TT and CT mismatches at this position are cleaved at a low rate (similar to 1%), mismatches with other bases (such as GA and AC) and Watson-Crick base pairs are not cleaved by the enzyme. There is also no cleavage when the mismatched T is replaced with difluorotoluene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:09:29