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Titolo:
Complementation of defective translesion synthesis and UV light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase eta
Autore:
Yamada, A; Masutani, C; Iwai, S; Hanaoka, F;
Indirizzi:
Osaka Univ, Inst Mol & Cellular Biol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ar Biol, Suita, Osaka 5650871, Japan Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 eut Sci, Suita, Osaka 5650871, Japan Japan Sci & Technol Corp, CREST, Suita, Osaka 5650871, Japan Japan Sci & Technol Corp Suita Osaka Japan 5650871 , Osaka 5650871, Japan RIKEN, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198RIKEN, Wako, Saitama 3510198, Japan Biomol Engn Res Inst, Suita, Osaka 5650874, Japan Biomol Engn Res Inst Suita Osaka Japan 5650874 uita, Osaka 5650874, Japan
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 13, volume: 28, anno: 2000,
pagine: 2473 - 2480
SICI:
0305-1048(20000701)28:13<2473:CODTSA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI DINB; THYMINE-THYMINE DIMER; CATALYTIC SUBUNIT; MOLECULAR-CLONING; GENE-EXPRESSION; MESSENGER-RNA; REPLICATION; BYPASS; MUTAGENESIS; PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Hanaoka, F Osaka Univ, Inst Mol & Cellular Biol, 1-3 Yamada Oka, Suita, Osaka 5650871, Japan Osaka Univ 1-3 Yamada Oka Suita Osaka Japan 5650871 0871, Japan
Citazione:
A. Yamada et al., "Complementation of defective translesion synthesis and UV light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase eta", NUCL ACID R, 28(13), 2000, pp. 2473-2480

Abstract

Defects in the human gene XPV result in the variant form of the genetic disease xeroderma pigmentosum (XP-V), XPV encodes DNA polymerase eta, a novelDNA polymerase that belongs to the UmuC/DinB/Rad30 superfamily, This polymerase catalyzes the efficient and accurate translesion synthesis of DNA past cis-syn cyclobutane di-thymine lesions. In this report we present the cDNA sequence and expression profiles of the mouse XPV gene and demonstrate its ability to complement defective DNA synthesis in XP-V cells. The mouse XPV protein shares 80.3% amino acid identity and 86.9% similarity with the human XPV protein, The recombinant mouse XPV protein corrected the inability of XP-V cell extracts to carry out DNA replication, by bypassing thymine dimers on template DNA. Transfection of the mouse or human XPV cDNA into human XP-V cells corrected UV sensitivity. Northern blot analysis revealed thatthe mouse XPV gene is expressed ubiquitously, but at a higher level in testis, liver, skin and thymus compared to other tissues. Although the mouse XPV gene was not induced by UV irradiation, its expression was elevated similar to 4-fold during cell proliferation. These results suggest that DNA polymerase eta plays a role in DNA replication, though the enzyme is not essential for viability.

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Documento generato il 29/03/20 alle ore 15:13:44