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Titolo:
Development of a chromosome-plasmid balanced lethal system for Lactobacillus acidophilus with thyA gene as selective marker
Autore:
Fu, XL; Xu, JG;
Indirizzi:
Chinese Acad Prevent Med, Inst Epidemiol & Microbiol, Dept Microbiol, Beijing 102206, Peoples R China Chinese Acad Prevent Med Beijing Peoples R China 102206 Peoples R China
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 7, volume: 44, anno: 2000,
pagine: 551 - 556
SICI:
0385-5600(2000)44:7<551:DOACBL>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMIDYLATE SYNTHASE GENE; LACTIC-ACID BACTERIA; ESCHERICHIA-COLI; ANTIBIOTIC-RESISTANCE; LACTOCOCCUS-LACTIS; CONSTRUCTION; CLONING; VECTOR; DNA; STREPTOCOCCI;
Keywords:
Lactobacillus acidophilus; thymidylate synthase (thyA) gene; vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Xu, JG Chinese Acad Prevent Med, Inst Epidemiol & Microbiol, Dept Microbiol, Beijing 102206, Peoples R China Chinese Acad Prevent Med Beijing Peoples R China 102206 s R China
Citazione:
X.L. Fu e J.G. Xu, "Development of a chromosome-plasmid balanced lethal system for Lactobacillus acidophilus with thyA gene as selective marker", MICROB IMMU, 44(7), 2000, pp. 551-556

Abstract

A chromosome-plasmid balanced lethal gene delivery system for Lactobacillus acidophilus based on the thyA gene was developed. The selected L, acidophilus DOM La strain carries a mutated thyA gene and has an obligate requirement for thymidine, This strain can be used as a host for the constructed shuttle vector pFXL03, lacking antibiotic-resistant markers but having the mild-type thyA gene from L, casei which complements the thyA chromosomal mutation. The vector also contains the replicon region from plasmid pUC19 and that of the Lactococcus plasmid pWV01, which allows the transfer between Escherichia coli, L, casei and L, acidophilus, Eight unique restriction sites (i,e,, PstI, HindIII, SphI, San, ACCI, XbaI, KpnI and SacI) are available for cloning. After 40-time transfers in modified MRS medium, no plasmid losswas observed. The vector pFXL03 is potentially useful as a food-grade vaccine delivery system for L, acidophilus.

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Documento generato il 29/11/20 alle ore 17:31:23