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Titolo:
Ectodomain of coxsackievirus and adenovirus receptor genetically fused to epidermal growth factor mediates adenovirus targeting to epidermal growth factor receptor-positive cells
Autore:
Dmitriev, I; Kashentseva, E; Rogers, BE; Krasnykh, V; Curiel, DT;
Indirizzi:
Univ Alabama, Gene Therapy Ctr, Dept Med, Div Human Gene Therapy, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ne Therapy, Birmingham, AL 35294 USA Univ Alabama, Gene Therapy Ctr, Dept Pathol, Div Human Gene Therapy, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ne Therapy, Birmingham, AL 35294 USA Univ Alabama, Gene Therapy Ctr, Dept Surg, Div Human Gene Therapy, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ne Therapy, Birmingham, AL 35294 USA Univ Alabama, Dept Radiat Oncol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 diat Oncol, Birmingham, AL 35294 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 15, volume: 74, anno: 2000,
pagine: 6875 - 6884
SICI:
0022-538X(200008)74:15<6875:EOCAAR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TRANSFER; TYPE-5 FIBER; MUSCLE-CELLS; CELLULAR RECEPTORS; AIRWAY EPITHELIA; DENDRITIC CELLS; PENTON BASE; SUBGROUP-C; B VIRUSES; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Curiel, DT Univ Alabama, Gene Therapy Ctr, Dept Med, Div Human Gene Therapy, 1824 6thAve S,Room WTI 620, Birmingham, AL 35294 USA Univ Alabama 1824 6th Ave S,Room WTI 620 Birmingham AL USA 35294
Citazione:
I. Dmitriev et al., "Ectodomain of coxsackievirus and adenovirus receptor genetically fused to epidermal growth factor mediates adenovirus targeting to epidermal growth factor receptor-positive cells", J VIROLOGY, 74(15), 2000, pp. 6875-6884

Abstract

Human adenovirus (Ad) is extensively used for a variety of gene therapy applications. However, the utility of Ad vectors is limited due to the low efficiency of Ad-mediated gene transfer to target cells expressing marginal levels of the Ad fiber receptor. Therefore, the present generation of Ad vectors could potentially be improved by modification of Ad tropism to target the virus to specific organs and tissues. The fact that coxsackievirus and adenovirus receptor (CAR) does not play any role in virus internalization, but functions merely as the virus attachment site, suggests that the extracellular part of CAR might be utilized to block the receptor recognition site on the Ad fiber knob domain, We proposed to design bispecific fusion proteins formed by a recombinant soluble form of truncated CAR (sCAR) and a targeting ligand. In this study, we derived sCAR genetically fused with human epidermal growth factor (EGF) and investigated its ability to target Ad infection to the EGF receptor (EGFR) overexpressed on cancer cell lines. We have demonstrated that sCAR-EGF protein is capable of binding to Ad virions and directing them to EGFR, thereby achieving targeted delivery of reporter gene. These results show that sCAR-EGF protein possesses the ability to effectively retarget Ad via a non-CAR pathway, with enhancement of gene transfer efficiency.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:24:09