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Titolo:
Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon)
Autore:
Yamauchi, K; Hamasaki, S; Miyazaki, K; Kikusui, T; Takeuchi, Y; Mori, Y;
Indirizzi:
Univ Tokyo, Lab Vet Ethol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 et Ethol, Bunkyo Ku, Tokyo 1138657, Japan Wildlife Management Off, Tama Ku, Kawasaki, Kanagawa 2140011, Japan Wildlife Management Off Kawasaki Kanagawa Japan 2140011 wa 2140011, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 6, volume: 62, anno: 2000,
pagine: 669 - 671
SICI:
0916-7250(200006)62:6<669:SDBOFD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PCR;
Keywords:
amelogenin; feces; sex determination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Mori, Y Univ Tokyo, Lab Vet Ethol, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657 , Tokyo 1138657, Japan
Citazione:
K. Yamauchi et al., "Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon)", J VET MED S, 62(6), 2000, pp. 669-671

Abstract

A Sex determination method using DNA extracted from feces has been developed for sika deer (Cervus nippon). We determined a partial sequence of the amelogenin gene of sika deer, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. Based on the sexually dimorphic sequences, we designed a pair of primers which could amplify DNA fragments the lengths of which are different between males and females. PCR products weredetected in 34 out of 37 fecal samples collected from captured deer and the sexes estimated by the present method were perfectly matched with the actual sexes. feces, sex determination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 10:14:41