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Titolo:
Renin angiotensin system of rabbit clitoral cavernosum: Interaction with nitric oxide
Autore:
Park, JK; Kim, SZ; Kim, SH; Kim, YG; Cho, KW;
Indirizzi:
Chonbuk Natl Univ, Sch Med, Dept Urol, Chonju 560180, South Korea Chonbuk Natl Univ Chonju South Korea 560180 , Chonju 560180, South Korea Chonbuk Natl Univ, Sch Med, Dept Physiol, Chonju 560180, South Korea Chonbuk Natl Univ Chonju South Korea 560180 , Chonju 560180, South Korea Chonbuk Natl Univ, Inst Med Sci, Chonju 560180, South Korea Chonbuk Natl Univ Chonju South Korea 560180 , Chonju 560180, South Korea
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 2, volume: 164, anno: 2000,
pagine: 556 - 561
SICI:
0022-5347(200008)164:2<556:RASORC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
II RECEPTOR SUBTYPES; FEMALE SEXUAL DYSFUNCTION; CORPUS CAVERNOSUM; SMOOTH-MUSCLE; ERECTILE INSUFFICIENCY; VAGINAL ENGORGEMENT; RAT; TISSUE; WOMEN; MODULATOR;
Keywords:
clitoris; angiotensin II; nitric oxide; autoradiography; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Park, JK Chonbuk Natl Univ, Sch Med, Dept Urol, 2-20 Keum Am Dong San, Chonju 560180, South Korea Chonbuk Natl Univ 2-20 Keum Am Dong San Chonju South Korea 560180
Citazione:
J.K. Park et al., "Renin angiotensin system of rabbit clitoral cavernosum: Interaction with nitric oxide", J UROL, 164(2), 2000, pp. 556-561

Abstract

Purpose: Angiotensin (ANG) II has been known to be a potent modulator for the maintenance of smooth muscle tone of the penile cavernosum. However, its role in clitoral cavernosum is unknown. The clitoris is the homologue of the penis arising from the embryological genital tubercle. We investigated the presence of ANG II receptors, the function of ANG II, and its interaction with nitric oxide (NO) in rabbit clitoral cavernosum. Materials and Methods: The isometric tension was measured in the strips ofclitoral cavernosum. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate expression of AT1a and AT1b ANG II receptor subtype mRNAs. In vitro autoradiography was used to localize ANG II receptors in the clitoral cavernosum. Results: The clitoral cavernosum was contracted dose-dependently by the addition of ANG II. Dup 753 (ANG II type 1 receptor antagonist) inhibited significantly ANG II induced contraction. PD 123,319 (ANG II type 2 receptor antagonist) did not affect the ANG II response. Pretreatment with N-G-nitro-L-arginine methyl ester (NO synthase inhibitor) accentuated contractions induced by ANG II. Specific binding sites for I-125-ANG II were found in the clitoral cavernosum, The dissociation constant (K-d) was 0.58 +/- 0.05 nM. Specific binding of I-125-ANG II was displaced by Dup 753 (10(-5) M) but not by PD 123,319 (10-5 M). The inhibitory constant (Ki) for Dup 753 was 23.4+/- 9.73 nM and mRNAs for AT1a and AT1b receptor subtypes were detected byRT-PCR. Conclusion: The present study shows that ANG II is involved in the regulation of clitoral cavernosum smooth muscle tone via ANG II receptor subtype AT1, and that ANG II has cross-talk with NO.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:23:09