Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Thrombopoietin enhances neutrophil production by bone marrow hematopoieticprogenitors with the aid of stem cell factor in congenital neutropenia
Autore:
Sawai, N; Koike, K; Mwamtemi, HH; Ito, S; Kurokawa, Y; Sakashita, K; Kinoshita, T; Higuchi, T; Takeuchi, K; Shiohara, M; Kamijo, T; Higuchi, Y; Miyazaki, H; Kato, T; Kobayashi, M; Miyake, M; Yasui, K; Komiyama, A;
Indirizzi:
Shinshu Univ, Sch Med, Dept Pediat, Matsumoto, Nagano 3908621, Japan Shinshu Univ Matsumoto Nagano Japan 3908621 sumoto, Nagano 3908621, Japan Shinshu Univ Hosp, Blood Transfus Serv, Matsumoto, Nagano, Japan Shinshu Univ Hosp Matsumoto Nagano Japan Serv, Matsumoto, Nagano, Japan Shinshu Univ Hosp, Cent Clin Labs, Matsumoto, Nagano, Japan Shinshu Univ Hosp Matsumoto Nagano Japan Labs, Matsumoto, Nagano, Japan Kirin Brewery Co Ltd, Pharmaceut Res Lab, Takasaki, Gumma, Japan Kirin Brewery Co Ltd Takasaki Gumma Japan es Lab, Takasaki, Gumma, Japan Hiroshima Univ, Sch Med, Dept Pediat, Hiroshima, Japan Hiroshima Univ Hiroshima Japan , Sch Med, Dept Pediat, Hiroshima, Japan Osaka Med Coll, Dept Pediat, Takatsuki, Osaka 569, Japan Osaka Med Coll Takatsuki Osaka Japan 569 iat, Takatsuki, Osaka 569, Japan
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 1, volume: 68, anno: 2000,
pagine: 137 - 143
SICI:
0741-5400(200007)68:1<137:TENPBB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING-FACTOR; ACUTE MYELOID-LEUKEMIA; CORD-BLOOD-CELLS; FACTOR G-CSF; FACTOR-RECEPTOR; MYELOSUPPRESSED MICE; DIFFERENTIATION; MEGAKARYOCYTES; APOPTOSIS; RECOVERY;
Keywords:
granulocyte colony-stimulating factor; CD34(+) CD38(+) c-kit(+) cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Koike, K Shinshu Univ, Sch Med, Dept Pediat, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan Shinshu Univ 3-1-1 Asahi Matsumoto Nagano Japan 3908621 21, Japan
Citazione:
N. Sawai et al., "Thrombopoietin enhances neutrophil production by bone marrow hematopoieticprogenitors with the aid of stem cell factor in congenital neutropenia", J LEUK BIOL, 68(1), 2000, pp. 137-143

Abstract

We examined the effects of granulocyte colony-stimulating factor (G-CSF), stent cell factor (SCF), and thrombopoietin (TPO), alone or in combination,on the generation of neutrophils by bone marrow (BM) cells from three patients with severe congenital neutropenia (SCN) through the use of a serum-deprived Liquid culture system. Synergistic effects of G-CSF and SCF on the neutrophil production by BM CD34(+)CD38(+)c-kit(+) cells were observed in SCN patients as well as in normal controls. The addition of TPO to the culture containing G-CSF and SCF further augmented the growth of neutrophils in the two groups, Single-cell culture experiments revealed that the three-factor combination caused increases in both. the number and size of neutrophil colonies compared with G-CSF + SCF in normal BM cells, whereas only a significant increment in the colony size was observed in SCN patients. Even in the presence of SCF or SCF + TPO, the concentrations of G-CSF necessary for the substantial production of neutrophils by CD39(+)CD38(+)c-kit(+) cells were higher in two patients compared with the levels obtained by normal control cells. In addition, TPO did not accelerate the maturation of neutrophilic cells supported by G-CSF + SCF, When BM CD34(+)CD38(-)c-kit(+) cells were targeted, the addition of TPO to the culture containing G-CSF and SCF wasrequired for significant neutrophil colony growth in the two groups. Theseresults suggest that TPO enhances the G-CSF-dependent neutrophil production with the aid of SCF in this disorder.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:19:24