Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Erythropoietin-inducible immediate-early genes in human vascular endothelial cells
Autore:
Fodinger, M; Fritsche-Polanz, R; Buchmayer, H; Skoupy, S; Sengoelge, G; Horl, WH; Sunder-Plassmann, G;
Indirizzi:
Univ Vienna, Dept Lab Med, Div Mol Biol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 , Div Mol Biol, A-1090 Vienna, Austria Univ Vienna, Dept Internal Med 3, Div Nephrol & Dialysis, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 rol & Dialysis, A-1090 Vienna, Austria
Titolo Testata:
JOURNAL OF INVESTIGATIVE MEDICINE
fascicolo: 2, volume: 48, anno: 2000,
pagine: 137 - 149
SICI:
1081-5589(200003)48:2<137:EIGIHV>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT-HUMAN-ERYTHROPOIETIN; PROTEIN-TYROSINE-PHOSPHATASE; TRANSFER-RNA-SYNTHETASE; RAT CARDIAC ALLOGRAFTS; CHRONIC-RENAL-FAILURE; PTP-PEST; C-MYC; PREPROENDOTHELIN-1 GENE; ADP-RIBOSYLTRANSFERASE; FUNCTIONAL-PROPERTIES;
Keywords:
endothelial cells; recombinant human erythropoietin; gene expression; immediate-early genes; differential display;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
97
Recensione:
Indirizzi per estratti:
Indirizzo: Fodinger, M Univ Vienna, Dept Lab Med, Div Mol Biol, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 tria
Citazione:
M. Fodinger et al., "Erythropoietin-inducible immediate-early genes in human vascular endothelial cells", J INVES MED, 48(2), 2000, pp. 137-149

Abstract

Background: Patients receiving recombinant human erythropoietin (rHuEPO) may experience side effects arising from the vascular system, The underlyingmechanisms, however, are largely unknown. Methods: To elucidate downstream events following erythropoietin receptor triggering, a differential display analysis of human vascular endothelial cell mRNA mas performed,Results: We identified eight genes that mere upregulated by rHuEPO as confirmed in two further independent cell culture experiments using a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) protocol. The genes coded for proteins that may be assigned to four different groups:1) proteins implicated in the regulation of vascular functions (thrombospondin-1, 20 kDa myosin regulatory light chain; relative increase of rHuEPO induced mRNA levels: 155.2%, P=0.043; 137.6%, P=0.036, respectively); 2) gene products involved in gene transcription and/or translation (c-myc purine-binding transcription factor PuF, tryptophanyl-tRNA synthetase, S19 ribosomal protein; increase of mRNA levels: 126.4%, P=0.032; 150.9%, P=0.012; 134.9%, P=0.038); 3) subunits of mitochondrial enzymes related to energy transfer (NADH dehydrogenase subunit 6, cytochrome C oxidase subunit 1; increase of mRNA concentrations: 141.7%, P=0.007; 140.3%, P=0.01); and 4) regulatorsof signal transduction (protein tyrosine phosphatase G1, increase of transcript level: 160.3%, P=0.016),Conclusions: We report on novel molecular downstream events following rHuEPO receptor triggering of human vascular endothelial cells, We identified EPO-responsive immediate-early genes, coding for proteins involved in vascular functions, gene transcription, and/or translation, energy transfer, and signal transduction, Thus, our data provide new insights into the molecularchanges induced by EPO in human vascular endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 13:29:30