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Titolo:
A role for the region encompassing the c '' strand of a TCR V alpha domainin T cell activation events
Autore:
Qadri, A; Radu, CG; Thatte, J; Cianga, P; Ober, BT; Ober, RJ; Ward, ES;
Indirizzi:
Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas, Dept Elect Engn, Richardson, TX 75083 USA Univ Texas Richardson TX USA 75083 t Elect Engn, Richardson, TX 75083 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 2, volume: 165, anno: 2000,
pagine: 820 - 829
SICI:
0022-1767(20000715)165:2<820:ARFTRE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAS-LIGAND EXPRESSION; PEPTIDE-MHC COMPLEXES; MYELIN BASIC-PROTEIN; CLASS-II MHC; LYMPHOCYTE ANTIGEN RECEPTORS; SIGNAL-TRANSDUCTION; DOWN-REGULATION; ANTAGONIST LIGANDS; AUTOIMMUNE ENCEPHALOMYELITIS; MOLECULAR REQUIREMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
91
Recensione:
Indirizzi per estratti:
Indirizzo: Ward, ES Univ Texas, SW Med Ctr, Ctr Immunol, 5323 Harry Hines Blvd, Dallas, TX 75235 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 TX 75235 USA
Citazione:
A. Qadri et al., "A role for the region encompassing the c '' strand of a TCR V alpha domainin T cell activation events", J IMMUNOL, 165(2), 2000, pp. 820-829

Abstract

The distinct strand topology of TCR V alpha domains results in a flatter surface in the region encompassing the c " strand than the corresponding region in Ig V domains. In the current study a possible role for this region in T cell activation has been investigated by inserting a potential glycosylation site at V alpha residue 82, This residue is in proximity to the c " strand and distal to the putative interaction site for cognate peptide:MHC ligand, An additional N-linked carbohydrate at this position would create a protrusion on the V alpha domain surface, and this may interfere with TCR aggregation and/or recruitment of signaling molecules. The modified TCR has been expressed in transfected T cells, and the phenotype following stimulation has been compared with that of cells expressing the wild-type TCR, The mutation has significant effects on activation-induced cell death and TCR internalization, but, unexpectedly, does not affect IL-2 secretion, Furthermore, analyses with tetrameric, peptide:MHC class II complexes suggest that the mutation decreases the ability of the TCR to aggregate into a configuration compatible with avid binding by these multivalent ligands.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:52:42