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Titolo:
Solid- and Liquid-phase extraction for the gas chromatographic-tandem massspectrometric quantification of 2,3-dinor-thromboxane B-2 and 2,3-dinor-6-oxo-prostaglandin F-1 alpha in human urine
Autore:
Tsikas, D; Gutzki, FM; Bohme, M; Fuchs, I; Frolich, JC;
Indirizzi:
Hannover Med Sch, Inst Clin Pharmacol, D-30623 Hannover, Germany Hannover Med Sch Hannover Germany D-30623 col, D-30623 Hannover, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 885, anno: 2000,
pagine: 351 - 359
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT URINE; IN-VIVO; THROMBOXANE-B2; METABOLISM; IDENTIFICATION;
Keywords:
thromboxanes; prostaglandins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Tsikas, D Hannover Med Sch, Inst Clin Pharmacol, Carl Neuberg Str 1, D-30623 Hannover, Germany Hannover Med Sch Carl Neuberg Str 1 Hannover Germany D-30623 ny
Citazione:
D. Tsikas et al., "Solid- and Liquid-phase extraction for the gas chromatographic-tandem massspectrometric quantification of 2,3-dinor-thromboxane B-2 and 2,3-dinor-6-oxo-prostaglandin F-1 alpha in human urine", J CHROMAT A, 885(1-2), 2000, pp. 351-359

Abstract

Whole body synthesis of thromboxane A(2) is best assessed by quantifying non-invasively its major urinary metabolite, i.e., 2,3-dinor-thromboxane B-2(2,3-dn-TxB(2)), by gas chromatography-mass spectrometry (GC-MS) or GC-tandem MS. Methods based on these techniques usually require a series of extraction and purification procedures including solid-phase extraction (SPE) and thin-layer chromatography (TLC) or liquid chromatographic separation of authentic or derivatized 2,3-dn-TxB(2). Taking advantage of the inherent accuracy of GC-tandem MS and the high selectivity of the extraction of methoximated 2,3-dn-TxB(2) on phenylboronic acid SPE cartridges we developed a method that involves only SPE steps prior to quantification by GC-tandem MS. The method was validated by performing in Parallel an additional TLC step. Method mean accuracy and precision were of the order of 103% and 95%, respectively. The method allows furthermore co-processing of the same urine sample to quantify accurately and rapidly the major urinary metabolite of prostacyclin, i.e., 2,3-dn-6-oxo-prostaglandin (PG) F-1 alpha, by GC-tandem MS. The limit of detection of the method was below each 5 pg of 2,3-dn-TxB(2) and 2,3-dn-6-oxo-PGF(1 alpha) per 5 ml of urine. Our study suggests that diner metabolites of isothromboxanes and isoprostacyclins are not abundantly present in human urine. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 12/07/20 alle ore 06:38:57