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Titolo:
PHARMACOKINETIC ANALYSIS OF FREE-RADICALS BY IN-VIVO BCM (BLOOD-CIRCULATION MONITORING) ESR METHOD
Autore:
TAKECHI K; TAMURA H; YAMAOKA K; SAKURAI H;
Indirizzi:
KYOTO PHARMACEUT UNIV,DEPT ANALYT & BIOINORGAN CHEM,NAKAUCHI CHO 5 KYOTO 607 JAPAN KYOTO PHARMACEUT UNIV,DEPT ANALYT & BIOINORGAN CHEM KYOTO 607 JAPAN KYOTO UNIV,FAC PHARMACEUT SCI,DEPT ANALYT CHEM,SAKYO KU KYOTO 606 JAPAN
Titolo Testata:
Free radical research
fascicolo: 6, volume: 26, anno: 1997,
pagine: 483 - 496
SICI:
1071-5762(1997)26:6<483:PAOFBI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-SPIN-RESONANCE; NITROXIDE RADICALS; ASCORBIC-ACID; WHOLE MOUSE; RAT-LIVER; IN-VIVO; REDUCTION; LABELS; SPECTROMETRY; METABOLISM;
Keywords:
X-BAND ESR; BLOOD CIRCULATION MONITORING; NITROXIDE RADICALS; RAT; PHARMACOKINETICS; RADICAL CLEARANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
K. Takechi et al., "PHARMACOKINETIC ANALYSIS OF FREE-RADICALS BY IN-VIVO BCM (BLOOD-CIRCULATION MONITORING) ESR METHOD", Free radical research, 26(6), 1997, pp. 483-496

Abstract

In pharmacokinetic studies, a variety of analytical method including radioisotopic detection and HPLC (high performance liquid chromatography) has been used. In the present investigation, we developed in vivo BCM (Blood Circulation Monitoring)-ESR method, which is a new technique with a conventional X-band ESR spectrometer for observing stable free radicals in the circulating blood of living rats under anaes thesia. Both 5-(PROXYL derivatives) and 6-(TEMPO derivatives) membered nitroxide spin probes with various types of substituent functional group were used. After physico-chemical properties of the spin probes such as hyperfine coupling constant (A-value), g-value and partition coefficient as well as chemical stability of the compounds in the fresh blood were obtained, the in vivo BCM-ESR method was performed in normal rats. Several pharmacokinetic parameters such as half-life of the probes, distribution volume, total body clearance and mean residence time were obtained and discussed in terms of their chemical structures. In addition, clearance of a spin probe was related to the urine concentration. The BCM-ESR method was found to be very useful to observe free radicals at the real time. By time-dependent ESR signal decay of spin probes,pharmacokinetic parameters were obtained.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 04:32:04