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Titolo:
Retroviral expression of Wnt-1 and Wnt-7b produces different effects in mouse mammary epithelium
Autore:
Naylor, S; Smalley, MJ; Robertson, D; Gusterson, BA; Edwards, PAW; Dale, TC;
Indirizzi:
Inst Canc Res, Toby Robins Breakthrough Breast Canc Res Ctr, Sect Cell Biol & Expt Pathol, London SW3 6JB, England Inst Canc Res London England SW36JB xpt Pathol, London SW3 6JB, England Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP hol, Cambridge CB2 1QP, England
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 12, volume: 113, anno: 2000,
pagine: 2129 - 2138
SICI:
0021-9533(200006)113:12<2129:REOWAW>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; GENE-EXPRESSION; SIGNAL-TRANSDUCTION; WINGLESS PROTEIN; TRANSGENIC MICE; AXIS FORMATION; GROWTH-FACTOR; CELL-SURFACE; IN-VITRO; GLAND;
Keywords:
Wnt gene; mammary gland; retroviral gene transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Dale, TC Inst Canc Res, Toby Robins Breakthrough Breast Canc Res Ctr, SectCell Biol & Expt Pathol, 237 Fulham Rd, London SW3 6JB, England Inst Canc Res 237 Fulham Rd London England SW3 6JB 6JB, England
Citazione:
S. Naylor et al., "Retroviral expression of Wnt-1 and Wnt-7b produces different effects in mouse mammary epithelium", J CELL SCI, 113(12), 2000, pp. 2129-2138

Abstract

Several Wnt genes are expressed in the postnatal mouse mammary gland and are thought to be involved in mammary gland development. Ectopic expression of Wnt-1, which is not normally expressed in the mammary gland, drives the formation of a pre-neoplastic hyperplasia, Cell culture-based assays have shown that Wnt-1 and some mammary-expressed Wnts transform C57MG cells. Thishas led to the suggestion that Wnt-1 functions as an oncogene through the inappropriate activation of developmental events that are normally controlled by the 'transforming' class of Wnts. In this study, Wnt-7b was expressedin vivo using recombinant retroviruses, Wnt-7b did not alter normal mammary gland development despite having similar effects to Wnt-1 in cell culture. We conclude that the in vitro classification of Wnts as 'transforming' does not correlate with the transformation in vivo. To facilitate the analysis of Wnt-expression, a lacZ-containing, bicistronic recombinant retrovirus was developed. Immunohistochemistry and electron microscopy identified retrovirally transduced myoepithelial and luminal epithelial cells in normal and hyperplastic tissues. The distribution of transduced cells in mammary outgrowths was consistent with current models of mammary stem cell identity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:52:42