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Titolo:
Transactivation of naturally occurring HIV-1 long terminal repeats by the JNK signaling pathway - The most frequent naturally occurring length polymorphism sequence introduces a novel binding site for AP-1 factors
Autore:
Chen, PF; Flory, E; Avots, A; Jordan, BWM; Kirchhoff, F; Ludwig, S; Rapp, UR;
Indirizzi:
Univ Wurzburg, Inst Med Strahlenkunde & Zellforsch, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 forsch, D-97078 Wurzburg, Germany Univ Erlangen Nurnberg, Inst Klin & Mol Virol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Wurzburg, Inst Pathol, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 Pathol, D-97078 Wurzburg, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 27, volume: 275, anno: 2000,
pagine: 20382 - 20390
SICI:
0021-9258(20000707)275:27<20382:TONOHL>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ACTIVATED PROTEIN-KINASE; T-CELL ACTIVATION; P38 MAP KINASE; SERUM RESPONSE ELEMENT; NF-KAPPA-B; C-JUN; TRANSCRIPTION FACTOR; GENE-EXPRESSION; TRANSDUCTION PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Flory, E Paul Ehrlich Inst, Abt Med Biotechnol, Paul Ehrlich Str 95, D-63225 Langen, Germany Paul Ehrlich Inst Paul Ehrlich Str 95 Langen Germany D-63225 any
Citazione:
P.F. Chen et al., "Transactivation of naturally occurring HIV-1 long terminal repeats by the JNK signaling pathway - The most frequent naturally occurring length polymorphism sequence introduces a novel binding site for AP-1 factors", J BIOL CHEM, 275(27), 2000, pp. 20382-20390

Abstract

To study the role of MAPK cascades in the regulation of naturally occurring human immunodeficiency virus type I long terminal repeats (HIV-1 LTRs), we analyzed several HIV-1 LTRs from patients at different stages of disease progression. One of these naturally occurring HIV-1 LTRs contains an insertion termed the most frequent naturally occurring length polymorphism (MFNLP) and exhibited high inducibility upon T cell activation. We found that theprotein kinase mixed lineage kinase 3/src-homology 3 domain-containing proline-rich kinase, a specific activator of the stress-activated protein kinase (SAPK)/JNK signaling pathway in T lymphocytes, induces high transcriptional activation of this promoter. Promoter inducibility is inhibited by the SAPK/JNK inhibitor, the JNK binding domain of the JNK interacting protein 1, and Tam-67 (N-terminal deletion mutant of c-Jun). In electrophoretic mobility shift assay, several protein complexes were found to bind to the MFNLPsequence in T cells. me identified AP-1 factors c-Fos and JunB as MFNLP-binding proteins, whose binding: is abolished by introducing point mutations in the 3'-half of the MFNLP sequence. Introduction of these point mutationsinto the MFNLP containing HIV-1 LTR reduced src-homology 3 domain-containing proline-rich kinase -mediated transactivation. These data indicate that the AP-1-like binding site in the MFNLP sequence gives rise to a higher inducibility of natural HIV-LTRs by the SAPK/JNR. signaling pathway.

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Documento generato il 22/09/20 alle ore 20:15:16