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Titolo:
Deletion of an insulator element by the mutation facet-strawberry in Drosophila melanogaster
Autore:
Vazquez, J; Schedl, P;
Indirizzi:
Univ Calif San Francisco, Sch Med, Dept Biochem & Biophys, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 t Mol Biol, Princeton, NJ 08544 USA
Titolo Testata:
GENETICS
fascicolo: 3, volume: 155, anno: 2000,
pagine: 1297 - 1311
SICI:
0016-6731(200007)155:3<1297:DOAIEB>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENHANCER BLOCKING ACTIVITY; CHROMATIN DOMAIN BOUNDARY; HAIRY-WING PROTEIN; NOTCH LOCUS; BITHORAX COMPLEX; CHROMOSOMAL DOMAINS; POLYTENE CHROMOSOME; PROMOTER ELEMENT; IN-VIVO; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Vazquez, J Univ Calif San Francisco, Sch Med, Dept Biochem & Biophys, Box 0448, San Francisco, CA 94143 USA Univ Calif San Francisco Box 0448 San Francisco CA USA 94143 SA
Citazione:
J. Vazquez e P. Schedl, "Deletion of an insulator element by the mutation facet-strawberry in Drosophila melanogaster", GENETICS, 155(3), 2000, pp. 1297-1311

Abstract

Eukaryotic chromosomes are thought to be subdivided into a series of structurally and functionally independent units. Critical to this hypothesis is the identification of insulator or boundary elements that delimit chromosomal domains. The properties of a Notch mutation, facet-strawberry (fa(swb)),suggest that this small deletion disrupts such a boundary element fa(swb) is located in the interband separating polytene band 3C7, which contains Notch, from the distal band 3C6. The fa(swb) mutation alters the structural organization of the chromosome by deleting the interband and fusing 3C7 with3C6. Genetic studies also suggest that fa(swb) compromises the functional autonomy of Notch by allowing the locus to become sensitive to chromosomal position effects emanating from distal sequences. In the studies reported here, we show that a DNA fragment spanning the fa(swb) region can insulate reporter transgenes against chromosomal position effects and can block enhancer-promoter interactions. Moreover, we find that insulating activity is dependent on sequences deleted in fa(swb). These results provide evidence that the element defined by the fa(swb) mutation corresponds to an insulator.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:13:00