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Titolo:
ASSESSMENT OF CLONALITY IN CUTANEOUS LYMPHOID INFILTRATES BY POLYMERASE CHAIN-REACTION ANALYSIS OF IMMUNOGLOBULIN HEAVY-CHAIN GENE REARRANGEMENT
Autore:
RITTER JH; WICK MR; ADESOKAN PN; FITZGIBBON JF; ZHU XP; HUMPHREY PA;
Indirizzi:
WUMC,BARNES JEWISH HOSP,3RD FLOOR,PETERS BLDG,1 BARNES HOSP PLAZA ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,LAUREN V ACKERMAN LAB SURG PATHOL ST LOUIS MO63130
Titolo Testata:
American journal of clinical pathology
fascicolo: 1, volume: 108, anno: 1997,
pagine: 60 - 68
SICI:
0002-9173(1997)108:1<60:AOCICL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMA; T-CELL POPULATIONS; B-CELL; FLOW-CYTOMETRY; LYMPHOPROLIFERATIVE DISORDERS; FOLLICULAR HYPERPLASIA; MALIGNANT-LYMPHOMA; PARAFFIN SECTIONS; PRIMER SELECTION; SCREENING METHOD;
Keywords:
POLYMERASE CHAIN REACTION; IMMUNOGLOBULIN GENE REARRANGEMENTS; LYMPHOMA; CUTANEOUS LYMPHOID HYPERPLASIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J.H. Ritter et al., "ASSESSMENT OF CLONALITY IN CUTANEOUS LYMPHOID INFILTRATES BY POLYMERASE CHAIN-REACTION ANALYSIS OF IMMUNOGLOBULIN HEAVY-CHAIN GENE REARRANGEMENT", American journal of clinical pathology, 108(1), 1997, pp. 60-68

Abstract

Determination of the biologic potential of cutaneous lymphoid infiltrates may be difficult by standard histologic or immunohistologic examination. The polymerase chain reaction (PCR) has been used to document clonal rearrangements of the immunoglobulin heavy chain gene in paraffin-embedded fixed tissues. To explore the value of PCR in evaluation of cutaneous lymphoid infiltrates, 93 archival nonmycotic lymphoid lesions (28 small or mixed lymphocytic lymphomas, 15 large cell lymphomas,40 benign infiltrates, and 10 with atypical features) were analyzed. These cases had been previously immunophenotyped on paraffin sections,and clinical follow-up from 7 to 20 years was gathered. DNA products were generated using a seminested PCR technique, separated by 5% polyacrylamide gel elec trophoresis, stained with ethidium bromide, and visualized under UV light Cases with a 100- to 120-base pair band were scored as positive. Of the 28 small or mixed cell lymphomas, 23 had a B-cell immunophenotype or consisted of a mixture of B and T cells; of these, seven (30%) demonstrated a monoclonal pattern, and three (13%) were indeterminate. Twelve large cell cases were B-cell or mixed; five (42%) of these were positive for a monoclonal band, while four (33%) were indeterminate. None of five T-cell small cell or three T-cell largecell lymphomas demonstrated a monoclonal band. In contrast, 39 of the40 benign cases were T-cell predominant or mixed lesions. Nevertheless, 18 of these 40 cases on initial testing suggested possible monoclonality. Six were indeterminate, and 12 demonstrated apparent monoclonalbands, of which four were reproducible on repeat testing. No histologic or clinical features of lymphoma were present in 17 of these 18 cases, suggesting that they represent false-positive results. Most of thelatter lesions showed sparse perivascular infiltrates, with very few B cells. This suggests that amplification of the immunoglobulin heavy chain gene from a small number of lymphocytes may produce a monoclonalband. In summary, PCR may provide adjunct information about clonalityin selected lymphoid skin lesions, but is rather insensitive in this setting. Such data must be carefully considered in the context of the histologic, immunohistologic, and clinical findings, particularly whenassessing sparse infiltrates, because of the potential for false-positive results.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:07:01