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Titolo:
Spatiotemporal distribution of zonulae adherens and associated actin bundles in both epithelium and fiber cells during chicken lens development
Autore:
Lo, WK; Shaw, AP; Paulsen, DF; Mills, A;
Indirizzi:
Morehouse Sch Med, Dept Anat, Atlanta, GA 30310 USA Morehouse Sch Med Atlanta GA USA 30310 , Dept Anat, Atlanta, GA 30310 USA
Titolo Testata:
EXPERIMENTAL EYE RESEARCH
fascicolo: 1, volume: 71, anno: 2000,
pagine: 45 - 55
SICI:
0014-4835(200007)71:1<45:SDOZAA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADHESION MOLECULE UVOMORULIN; A-CAM; JUNCTIONS; MORPHOGENESIS; EXPRESSION; CADHERINS; MEMBRANE;
Keywords:
adherens junction; zonula adherens; fascia adherens; actin bundle; N-cadherin; lens development; chicken embryo; electron microscopy; immunofluorescence; immunoblotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Lo, WK Morehouse Sch Med, Dept Anat, 720 Westview Dr SW, Atlanta, GA 30310USA Morehouse Sch Med 720 Westview Dr SW Atlanta GA USA 30310 30310 USA
Citazione:
W.K. Lo et al., "Spatiotemporal distribution of zonulae adherens and associated actin bundles in both epithelium and fiber cells during chicken lens development", EXP EYE RES, 71(1), 2000, pp. 45-55

Abstract

Zonulae adherens and associated actin bundles (ZA/AB) are believed to playa major role in epithelial folding and invagination during morphogenesis of neural tube and other vesicular structures. The lens morphogenesis is associated with the formation of the lens vesicle in which ZA/AB would be needed during the formation process. However, the existence of ZA/AB in the lens has never been established. In this study we report for the first time the existence of ZA/AB in both lens epithelium and fiber cells during embryonic development of chicken lens from E4 to E20. Light microscopy revealed contacts between the lens epithelium and primary fiber cells, and between thelens epithelium and secondary fiber cells at E4 and Ell, respectively. Thin-section electron microscopy consistently revealed ZA/AB near both the apical ends of lens epithelial cells and primary fiber cells at E4. This arrangement manifests as a parallel pair of belt-like ZA/AB along the epithelium-fiber interface. In semi-tangential sections, a continuous belt-like ZA/ABwas also evidenced in individual epithelial cells and fiber cells. Furthermore, the same ZA/AB arrangement was observed near both the apical ends of epithelial cells and secondary fiber cells at Ell. Besides ZA/AB, macular-type fasciae adherens were distributed regularly between epithelial cells. between primary fibers, between secondary fibers, and between epithelium andboth primary and secondary fibers. Immunofluorescence strongly and preferentially labeled N-cadherin at both the apical ends of lens epithelium and primary or secondary fibers at the corresponding ages. suggesting a direct association with the zonulae adherens. Also punctate N-cadherin labeling wascommonly seen along Various regions of primary and secondary fiber cells at different ages, and to a larger extent in the mature fibers of older lenses. This study suggests that: (1) ZA/AB located at the apices of lens epithelial cells may play a crucial role in the early stages of lens morphogenesis (e.g. lens vesicle formation); (2) ZA/AB of primary and secondary fiber cells originate from the epithelial cells during their elongation and differentiation: (3) owing to the restricted distribution of ZA/AB, abundant fasciae adherens are needed to maintain the structural stability of the epithelium and fiber cells during development and maturation: and (4) N-cadherin is the principle adhesion protein for both the zonulae adherens and fasciaeadherens in the lens. (C) 2000 Academic Press.

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Documento generato il 02/10/20 alle ore 01:12:17