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Titolo:
Kinetochore "memory" of spindle checkpoint signaling in lysed mitotic cells
Autore:
Campbell, MS; Daum, JR; Gersch, MS; Nicklas, RB; Gorbsky, GJ;
Indirizzi:
Univ Oklahoma, Hlth Sci Ctr, Dept Cell Biol, Oklahoma City, OK 73104 USA Univ Oklahoma Oklahoma City OK USA 73104 iol, Oklahoma City, OK 73104 USA Duke Univ, Dept Zool, Durham, NC 27706 USA Duke Univ Durham NC USA 27706Duke Univ, Dept Zool, Durham, NC 27706 USA Univ Oklahoma, Hlth Sci Ctr, Dept Cell Biol, Oklahoma City, OK USA Univ Oklahoma Oklahoma City OK USA Dept Cell Biol, Oklahoma City, OK USA
Titolo Testata:
CELL MOTILITY AND THE CYTOSKELETON
fascicolo: 2, volume: 46, anno: 2000,
pagine: 146 - 156
SICI:
0886-1544(200006)46:2<146:K"OSCS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLOSOME/ANAPHASE-PROMOTING COMPLEX; SISTER-CHROMATID SEPARATION; SACCHAROMYCES-CEREVISIAE; UNATTACHED KINETOCHORES; ANAPHASE INITIATION; CYCLE CHECKPOINT; FISSION YEAST; BUDDING YEAST; PROTEIN MAD2; MITOSIS;
Keywords:
cell cycle; centromere; mitosis; cell division; chromosome; kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Gorbsky, GJ Univ Oklahoma, Hlth Sci Ctr, Dept Cell Biol, Biomed Res Bldg,Room 266,975 NE 10th St, Oklahoma City, OK 73104 USA Univ Oklahoma Biomed Res Bldg,Room 266,975 NE 10th St Oklahoma City OK USA 73104
Citazione:
M.S. Campbell et al., "Kinetochore "memory" of spindle checkpoint signaling in lysed mitotic cells", CELL MOTIL, 46(2), 2000, pp. 146-156

Abstract

The spindle checkpoint prevents errors in mitosis. Cells respond to the presence of kinetochores that are improperly attached to the mitotic spindle by delaying anaphase onset. Evidence suggests that phosphorylations recognized by the 3F3/2 anti-phos-phoepitope antibody may be involved in the kinetochore signaling of the spindle checkpoint. Mitoric cells lysed in detergent in the absence of phosphatase inhibitors rapidly lose expression of the 3F3/2 phosphoepitope. However, when ATP is added to lysed and rinsed mitoticcytoskeletons, kinetochores become rephosphsrylated by an endogenous, bound kinase. Kinetochore rephosphorylation in vitro produced the same differential phosphorylation seen in appropriately fixed living cells. In chromosomes not yet aligned at the metaphase plate, kinetochores undergo rapid rephosphorylation, while those of fully congressed chromosomes are under-phosphorylated. However, latent 3F3/2 kinase activity is retained at kinetochores of cells at all stages of mitosis including anaphase. This latent activity is revealed when rephosphorylation reactions are carried out for extended times. The endogenous, kinetochore-bound kinase can be chemically inactivated. Remarkably, a soluble kinase activity extracted from mitotic cells also caused differential rephosphorylation of kinetochores whose endogenous kinase had been chemically inactivated. We suggest that, in vivo, microtubule attachment alters the kinetochore 3F3/2 phosphoprotein, causing it to resistphosphorylation. This kinetochore modification is retained after cell lysis, producing a "memory" of the in vivo phosphorylation state. (C) 2000 Wiley-Liss, Inc.

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Documento generato il 03/07/20 alle ore 00:38:43