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Titolo:
Retroviral transduction of T lymphocytes for suicide gene therapy in allogeneic stem cell transplantation
Autore:
Kuhlcke, K; Ayuk, FA; Li, Z; Lindemann, C; Schilz, A; Schade, UM; Fauser, AA; Zander, AR; Eckert, HG; Fehse, B;
Indirizzi:
EUFETS GMBH, D-55743 Idar Oberstein, Germany EUFETS GMBH Idar Oberstein Germany D-55743 55743 Idar Oberstein, Germany Univ Hosp Eppendorf, Hamburg, Germany Univ Hosp Eppendorf Hamburg Germany iv Hosp Eppendorf, Hamburg, Germany BMT Hosp, Idar Oberstein, Germany BMT Hosp Idar Oberstein GermanyBMT Hosp, Idar Oberstein, Germany Dept Bone Marrow Transplantat & Haematol Oncol, Idar Oberstein, Germany Dept Bone Marrow Transplantat & Haematol Oncol Idar Oberstein Germany y
Titolo Testata:
BONE MARROW TRANSPLANTATION
, volume: 25, anno: 2000, supplemento:, 2
pagine: S96 - S98
SICI:
0268-3369(200005)25:<S96:RTOTLF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL-BLOOD LYMPHOCYTES; FIBRONECTIN FRAGMENTS; VECTOR; DEPLETION; INFECTION;
Keywords:
gene therapy; retroviral vectors; T lymphocytes; graft-versus-host disease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Kuhlcke, K EUFETS GMBH, Vollmersbachstr 66, D-55743 Idar Oberstein, Germany EUFETS GMBH Vollmersbachstr 66 Idar Oberstein Germany D-55743
Citazione:
K. Kuhlcke et al., "Retroviral transduction of T lymphocytes for suicide gene therapy in allogeneic stem cell transplantation", BONE MAR TR, 25, 2000, pp. S96-S98

Abstract

Transplantation of suicide gene modified allogeneic T lymphocytes is an approach to prevent T cell mediated GVHD while preserving the 'graft-versus-leukemia' (GVL) effect of an allograft, A prerequisite for such a therapy isthe efficient transduction of T cells with suitable vectors. Since existing techniques allow only insufficient transduction of T cells, the development of more efficient gene transfer protocols into these cells is of great importance. We present here a protocol for the highly efficient transductionof human primary T cells at high densities (1 x 10(6) cells/ml) by retroviral infection. The presented protocol allowed us to obtain transduction rates of more than 70% of CD3(+) cells after two cycles of infection. It is based on the use of FBS-free media for both the production of retrovirus-containing supernatant, as well as the cultivation of the primary T cells. Since the protocol presented here works just as efficiently under large scale conditions, it may easily he adapted to clinical needs and 'good manufacturing practice' (GMP) standards.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:45:31