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Titolo:
A simple method for the purification of over-expressed extracellular sucrase of Zymomonas mobilis from a recombinant Escherichia coli
Autore:
Sangiliyandi, G; Gunasekaran, P;
Indirizzi:
Madurai Kamaraj Univ, Sch Biol Sci, Dept Microbial Technol, Madurai 625021, Tamil Nadu, India Madurai Kamaraj Univ Madurai Tamil Nadu India 625021 1, Tamil Nadu, India
Titolo Testata:
BIOTECHNOLOGY LETTERS
fascicolo: 13, volume: 22, anno: 2000,
pagine: 1059 - 1062
SICI:
0141-5492(200007)22:13<1059:ASMFTP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHANOL-PRODUCTION; LEVANSUCRASE; INVERTASE; BACILLUS; LEVAN; GENE;
Keywords:
over-expression; protein purification; sucrase; Zymomonas mobilis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Sangiliyandi, G Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 ehovot, Israel
Citazione:
G. Sangiliyandi e P. Gunasekaran, "A simple method for the purification of over-expressed extracellular sucrase of Zymomonas mobilis from a recombinant Escherichia coli", BIOTECH LET, 22(13), 2000, pp. 1059-1062

Abstract

The over-expressed extracellular sucrase (SacC) of Zymomonas mobilis from a recombinant Escherichia coli (pZSP62) carrying the sacC gene was purifiedpartially by repeated cycles of freezing and thawing. This method separated the highly expressed recombinant protein from the bulk of endogenous E. coli proteins. The enzyme was further purified 14 fold with a 55% yield fromthe cellular extract of E. coli by hydroxyapatite chromatography. The purified enzyme had a Mr of 46 kDa by SDS-PAGE. Its km value for sucrose was 86mM and was optimal at pH 5.0 and at 36 degrees C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:59:56