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Titolo:
Phenotypic modulation of nasal septal chondrocytes by cytoskeleton modification
Autore:
Loty, S; Sautier, JM; Forest, N;
Indirizzi:
Univ Paris 07, Inst Biomed Cordeliers, Fac Chirurg Dent, Lab Biol Odontol,F-75270 Paris 06, France Univ Paris 07 Paris France 06 Lab Biol Odontol,F-75270 Paris 06, France
Titolo Testata:
BIORHEOLOGY
fascicolo: 1-2, volume: 37, anno: 2000,
pagine: 117 - 125
SICI:
0006-355X(2000)37:1-2<117:PMONSC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; CHICK CHONDROCYTES; GENE-EXPRESSION; ALGINATE BEADS; CELL-SHAPE; COLLAGEN; CULTURE; DIFFERENTIATION; CHONDROGENESIS; PROTEOGLYCAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Loty, S Univ Paris 07, Inst Biomed Cordeliers, Fac Chirurg Dent, Lab Biol Odontol,15-21 Rue Ecole Med, F-75270 Paris 06, France Univ Paris 07 15-21 Rue Ecole Med Paris France 06 aris 06, France
Citazione:
S. Loty et al., "Phenotypic modulation of nasal septal chondrocytes by cytoskeleton modification", BIORHEOLOGY, 37(1-2), 2000, pp. 117-125

Abstract

The reversion to the initial round shape of chondrocytes in monolayer cultures is one of the initial events required for the expression of cartilage-specific macromolecules. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of culturedchondrocytes. Our study focuses on evaluating the response of dedifferentiated fetal rat chondrocytes to cytochalasin D, an actin-disrupting agent, with special emphasis on the morphological events. Immediately after exposure to the drug, cells round up but Batten again after removing the agent. However, immunocytochemical procedures revealed a disorganization of microfilaments and intermediate filaments. Phase-contrast and scanning electron microscopic observations revealed that on day 6 of culture, cells located at the top of the cell layer adopted a spherical morphology. Prominent differences were noted in control cultures where cells had to aggregate prior to overt chondrogenesis. These morphological changes occurred parallel to the expression of type II collagen, marker of the chondrocytes phenotype, strongly expressed in experimental cultures, but relatively weak in control cultures, and only restricted on areas of polygonal cellular aggregates. Furthermore, [S-35]-sulphate incorporation into sulphated glycosaminoglycans increased rapidly with the period of culture to a maximum after 7 days and was then two-fold in treated cultures. Taken together. these findings indicated that cytochalasin-D stimulates chondrogenesis in response to modification ofcytoskeleton architecture and the subsequent rounding up of the cells.

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Documento generato il 26/01/21 alle ore 04:42:55