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Titolo:
THE IN-VITRO EFFECTS OF INTERLEUKIN-12 UPON TUMOR-INFILTRATING LYMPHOCYTES DERIVED FROM RENAL-CELL CARCINOMA
Autore:
STEGER GG; GNANT MF; DJAVANMARD MP; MADER RM; JAKESZ R; PIERCE W; DEKERNION JB; FIGLIN R; BELLDEGRUN A;
Indirizzi:
UNIV VIENNA,DEPT INTERNAL MED 1,DIV ONCOL,WAHRINGER GURTEL 18-20 A-1090 VIENNA AUSTRIA UNIV VIENNA,DEPT SURG A-1090 VIENNA AUSTRIA UNIV CALIF LOS ANGELES,SCH MED,DEPT SURG,DIV UROL LOS ANGELES CA 90024 JONSSON COMPREHENS CANC CTR LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV HEMATOL ONCOL LOS ANGELESCA 90024
Titolo Testata:
Journal of cancer research and clinical oncology
fascicolo: 6, volume: 123, anno: 1997,
pagine: 317 - 324
SICI:
0171-5216(1997)123:6<317:TIEOIU>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATORY FACTOR NKSF; NECROSIS FACTOR-ALPHA; NATURAL-KILLER CELLS; T RESPONSES INVITRO; RECOMBINANT INTERLEUKIN-2; MATURATION FACTOR; INTERFERON-GAMMA; CYTOLYTIC ACTIVITY; PROLIFERATION; GENERATION;
Keywords:
INTERLEUKIN-12; TUMOR-INFILTRATING LYMPHOCYTES; RENAL CELL CANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
G.G. Steger et al., "THE IN-VITRO EFFECTS OF INTERLEUKIN-12 UPON TUMOR-INFILTRATING LYMPHOCYTES DERIVED FROM RENAL-CELL CARCINOMA", Journal of cancer research and clinical oncology, 123(6), 1997, pp. 317-324

Abstract

Clinical trials utilising interleukin (IL)-2 with tumor-infiltrating lymphocytes (TIL) have demonstrated efficacy in the treatment of metastatic renal cell carcinoma (RCC). Several cytokine's, as well as growth factors have demonstrated modulatory effects upon the biological properties of TIL from RCC, suggesting a potentially important role for cytokines other than IL-2 in the development of active and tumor-specific TIL. IL-12 was recently characterized as a natural-killer-cell-stimulatory factor or cytotoxic-T-cell-maturation factor. These propertiesof IL-12 prompted us to investigate the impact of this cytokine upon the activation of TIL from human RCC. In an attempt to enhance the in vitro growth and activity of renal TIL, we have grown eight renal TIL cultures in varying concentrations of IL-2 (8, 40, 80, 400 U/ml) and IL-12 (200 U/ml). In addition, IL-12 (200 U/ml) was added to TIL cultures pre-activated with IL-2 (400 U/ml). Growth, cell expansion, and theability of TIL to release certain cytokines upon tumor stimulation were determined. Proliferation assays, phenotypic analysis, and cytotoxicity assays were performed at an early and a late culture stage. IL-12, alone and when added to suboptimal concentrations of IL-2, failed toinduce TIL growth. While the addition of IL-12 to optimal doses of IL-2 suppressed TIL culture expansion, sequential culture exposure firstto IL-2 and then to IL-2 + IL-12 increased the number of cells expressing CD3(+)/CD56(+) and these cultures demonstrated enhanced in vitro lysis of autologous tumor. IL-12 clearly demonstrated a sequence-dependent impact of the biological behaviour of TIL from RCC. The optimal use of IL-12 in the in vitro expansion of renal TIL may result in cellswith an enhanced specific anti-tumor effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/06/20 alle ore 11:02:58