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Titolo:
Glomerular size and charge selectivity in the rat as revealed by FITC-Ficoll and albumin
Autore:
Ohlson, M; Sorensson, J; Haraldsson, B;
Indirizzi:
Univ Gothenburg, Dept Physiol, SE-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden SE-40530 , SE-40530 Gothenburg, Sweden Univ Gothenburg, Dept Nephrol, SE-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden SE-40530 , SE-40530 Gothenburg, Sweden
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 1, volume: 279, anno: 2000,
pagine: F84 - F91
SICI:
0363-6127(200007)279:1<F84:GSACSI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED KIDNEY PERFUSION; CAPILLARY WALL; ENDOGENOUS ALBUMIN; ENDOTHELIAL GLYCOCALYX; HORSERADISH-PEROXIDASE; PROXIMAL TUBULE; SERUM-ALBUMIN; PERMEABILITY; PERMSELECTIVITY; MACROMOLECULES;
Keywords:
capillary permeability; macromolecular transport; two-pore model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Ohlson, M Univ Gothenburg, Dept Physiol, Box 432, SE-40530 Gothenburg, Sweden Univ Gothenburg Box 432 Gothenburg Sweden SE-40530 burg, Sweden
Citazione:
M. Ohlson et al., "Glomerular size and charge selectivity in the rat as revealed by FITC-Ficoll and albumin", AM J P-REN, 279(1), 2000, pp. F84-F91

Abstract

The fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys in which the tubular activity was inhibited by low temperature (8 degrees C) and/or 10 mM NH4Cl. The Ficoll data were analyzed according to a two-pore model giving small and large pore radii of 46 Angstrom and 80-87 Angstrom, respectively. The estimated negative charge density was 35-45 meq/l at 8 degrees C. Perfusion with erythrocyte-free solutions of kidneys at 37 degrees C reduced glomerular size and charge perm-selectivity. Thus the large pore fraction of the glomerular filtrate (f(L)) was 1.64% at 37 degrees C compared with 0.94% at 8 degrees C. The theta for albumin was four times higher at 37 degrees C than at 8 degrees C (0.86% vs. 0.19%, respectively). NH4Cl caused further irreversible damage to the glomerular barrier. We conclude that there are no deleterious effects on the glomerular barrier of a reduction in temperature from 37 degrees C to8 degrees C. Therefore our data seem to disprove the hypothesis of low glomerular permselectivity and transtubular uptake of intact albumin and support the classic concept of a highly selective glomerular barrier.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 17:04:13