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Titolo:
Continuous infusion of cholecystokinin leads to down-regulation of the cholecystokinin-A receptor in the rat pancreas
Autore:
Ohlsson, B; Borg, K; Mulder, H; Rehfeld, JF; Axelson, J; Sundler, F;
Indirizzi:
Univ Lund, Dept Surg, Lund, Sweden Univ Lund Lund SwedenUniv Lund, Dept Surg, Lund, Sweden Univ Lund, Dept Physiol Sci, Sect Neuroendocrine Cell Biol, Lund, Sweden Univ Lund Lund Sweden Sci, Sect Neuroendocrine Cell Biol, Lund, Sweden Rigshosp, Dept Clin Biochem, DK-2100 Copenhagen, Denmark Rigshosp Copenhagen Denmark DK-2100 Biochem, DK-2100 Copenhagen, Denmark
Titolo Testata:
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
fascicolo: 6, volume: 35, anno: 2000,
pagine: 612 - 618
SICI:
0036-5521(200006)35:6<612:CIOCLT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TIME-COURSE; CELL; PHOSPHORYLATION; CERULEIN;
Keywords:
cholecystokinin; cholecystokinin-A receptor; pancreas; receptor regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Ohlsson, B Univ Hosp MAS, Dept Surg, SE-20502 Malmo, Sweden Univ Hosp MASMalmo Sweden SE-20502 g, SE-20502 Malmo, Sweden
Citazione:
B. Ohlsson et al., "Continuous infusion of cholecystokinin leads to down-regulation of the cholecystokinin-A receptor in the rat pancreas", SC J GASTR, 35(6), 2000, pp. 612-618

Abstract

Background: Infusion of sulphated cholecystokinin-8 (CCK-8S) in rats transiently increased the proliferation of pancreatic acinar cells, whereas the CCK-A receptor antagonist devazepide decreased such proliferation. This effect ceased after 3 days. CCK-8S or devazepide injected twice daily induced a persistent effect on the cell proliferation involving the major cells of the exocrine pancreas. The aim of this study was to examine the effect of continuous infusion of CCK-8S and devazepide on CCK-A receptor gene expression. Methods: Male Sprague-Dawley rats received subcutaneous continuous infusion of 5 mu g/kg/h CCK-8S, 200 mu g/kg/h devazepide, or 1% bovine serum albumin (BSA) by means of osmotic minipumps; The rats were killed after 3 days; 1 h before bring killed they received 5-broino-2-deoxyuridine (BrdU) intraperitoneally. Plasma was collected for analysis of CCK. The pancreas was dissected, and indirect immunofluorescence for BrdU and CCK-A receptor was performed. In situ hybridization to CCK-A receptor mRNA was performed for examination and semiquantification of receptor gene expression. Results: Continuous infusion of CCK-8S led to a sixfold increase in plasma CCK and a 40% increase in pancreatic weight. Devazepide did not affect the CCK level but decreased the pancreatic weight by 24% compared with BSA-infused rats. The BrdU labeling indicated that CCK-8S had no effect on cell proliferation. Immunofluorescence for the CCK-A receptor showed a decreased labeling intensity after CCK-8S infusion. The mean optical density of in situ hybridization labeling of the sections from CCK-8S-treated rats was decreased to 37% +/- 3% of that in controls. Devazepide did not affect the CCK-A receptor gene expression. Conclusions: Continuous stimulation of the CCK-B receptor ledto a downregulation of the receptor gene expression in pancreatic acinar cells and decreased labeling of the receptor at immunohistochemistry. The results suggest that down-regulation of the receptor is a protective mechanism against overstimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:01:52