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Titolo:
The F-actin cytoskeleton modulates slow secretory components rather than readily releasable vesicle pools in bovine chromaffin cells
Autore:
Gil, A; Rueda, J; Viniegra, S; Gutierrez, LM;
Indirizzi:
Univ Miguel Hernandez, CSIC, Ctr Mixto, Inst Neurociencias, Alicante 03550, Spain Univ Miguel Hernandez Alicante Spain 03550 encias, Alicante 03550, Spain Univ Miguel Hernandez, Fac Med, Dept Histol, Inst Neurociencias, Alicante 03550, Spain Univ Miguel Hernandez Alicante Spain 03550 encias, Alicante 03550, Spain Univ Miguel Hernandez, Fac Med, Ctr Cellular & Mol Biol, Alicante 03550, Spain Univ Miguel Hernandez Alicante Spain 03550 l Biol, Alicante 03550, Spain
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 98, anno: 2000,
pagine: 605 - 614
SICI:
0306-4522(2000)98:3<605:TFCMSS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; MYOSIN LIGHT-CHAIN; SYNAPTOSOME-ASSOCIATED PROTEIN; NEUROTRANSMITTER RELEASE; CATECHOLAMINE SECRETION; CONTRACTILE PROTEINS; CALCIUM CALMODULIN; CALYCULIN-A; EXOCYTOSIS; PHOSPHORYLATION;
Keywords:
exocytosis; amperometry; catecholamines; bovine adrenomedullary cells; cytoskeleton;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Gutierrez, LM Univ Miguel Hernandez, CSIC, Ctr Mixto, Inst Neurociencias, Campus San Juan, Alicante 03550, Spain Univ Miguel Hernandez Campus San Juan Alicante Spain 03550
Citazione:
A. Gil et al., "The F-actin cytoskeleton modulates slow secretory components rather than readily releasable vesicle pools in bovine chromaffin cells", NEUROSCIENC, 98(3), 2000, pp. 605-614

Abstract

Adrenal chromaffin cells were used to test the role of the peripheral cytoskeleton of F-actin in controlling different vesicle pools. Phorbol 12-myristate 13-acetate and calyculin A, two substances affecting phosphorylation-dephosphorylation cycles, produced different degrees of F-actin reorganization, inducing the partial and the almost total disassembly of this structure, respectively, as visualized using rhodamine-phalloidin staining. Consequently, electron microscopy studies revealed the higher efficiency of calyculin-A over phorbol 12-myristate 13-acetate in promoting vesicle access to the plasmalemma boundary. Surprisingly, only the phorbol ester enhanced fastkinetics and the population of rapidly releasable vesicle pools as studiedby single-cell amperometry, whereas both agents, as well as the F-actin severing compound, Latrunculin A, promoted an increase in the population of vesicles recruited in response to prolonged or repetitive stimulations. Taken together, our data support the notion that the F-actin peripheral barrier controls primary granule recruitment from reserve vesicle pools, whereas the phorbol ester effect on the rapidly releasable pools might be related to the alteration of late secretory stage through protein kinase C-dependent phosphorylation of an unidentified target. (C) 2000 IBRO. Published byElsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 18:46:01