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Titolo:
Expression pattern in a modified equalized kidney cDNA library of hypertensive rat
Autore:
Chern, TH; Chiang, FT; Wu, KD; Hsu, KL; Lo, HM; Tseng, CD; Tseng, YZ;
Indirizzi:
Natl Taiwan Univ, Coll Med, Dept Med, Taipei 10018, Taiwan Natl Taiwan Univ Taipei Taiwan 10018 Med, Dept Med, Taipei 10018, Taiwan Natl Yang Ming Univ, Sch Med, Inst Clin Med, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 Inst Clin Med, Taipei 112, Taiwan
Titolo Testata:
NEPHRON
fascicolo: 3, volume: 85, anno: 2000,
pagine: 258 - 266
SICI:
0028-2766(200007)85:3<258:EPIAME>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
WISTAR-KYOTO RATS; BLOOD-PRESSURE; CONSTRUCTION; HEART; GENE; CLONES;
Keywords:
hypertension; kidney genes; spontaneously hypertensive rat; modified equalized cDNA library;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Tseng, YZ Natl Taiwan Univ Hosp, Dept Internal Med, 7 Chung S Rd, Taipei 100, Taiwan Natl Taiwan Univ Hosp 7 Chung S Rd Taipei Taiwan 100 00, Taiwan
Citazione:
T.H. Chern et al., "Expression pattern in a modified equalized kidney cDNA library of hypertensive rat", NEPHRON, 85(3), 2000, pp. 258-266

Abstract

Background: Genes with important functions and rarely expressed would probably more easily be cloned from a modified equalized kidney cDNA library for further investigation. Methods: A kidney cDNA library of a spontaneously hypertensive rat was synthesized by a modified equalization method. Insertsof random clones were amplified by PCR and sequenced. Sequences were compared against a nonredundant database in GenBank. The cDNA profile was compared with an expression profile of a mouse renal proximal tubule cDNA library. Seven clones were analyzed by Northern blot analysis. The cDNA ends of two novel genes were amplified by PCR, sequenced and analyzed. Results: 336 cDNA clones were analyzed and grouped into 323 species of transcript with 77species similar to previously reported genes. Northern blot analysis identified one kidney-specific, one rarely expressed and lung-specific, and another relatively testis-specific gene. Two novel genes were cloned. One was 4.1 kb in length and encoded a 390-amino acid zinc-finger protein. Another was 2.5 kb and encoded a 474-amino acid protein of unknown function. Compared with the expression profile of a mouse renal proximal tubule cDNA library, this kidney library had a lower proportion of ribosomal genes and had a greater proportion of genes for signal transduction and DNA or RNA binding. Conclusions: Rare or novel genes could be more easily isolated from this library for molecular study of hypertension and renal pathophysiology,Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 04:16:09