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Titolo:
AN ENDOGENOUS DOPAMINERGIC NEUROTOXIN, N-METHYL-(R)-SALSOLINOL, INDUCES DNA-DAMAGE IN HUMAN DOPAMINERGIC NEUROBLASTOMA SH-SY5Y CELLS
Autore:
MARUYAMA W; NAOI M; KASAMATSU T; HASHIZUME Y; TAKAHASHI T; KOHDA K; DOSTERT P;
Indirizzi:
NAGOYA INST TECHNOL,DEPT BIOSCI,SHOWA KU NAGOYA AICHI 466 JAPAN NAGOYA INST TECHNOL,DEPT BIOSCI,SHOWA KU NAGOYA AICHI 466 JAPAN NATL INST LONGEV SCI,DEPT BASIC GERONTOL,BIOCHEM & METAB LAB OBU JAPAN NAGOYA CITY UNIV,FAC PHARMACEUT SCI NAGOYA AICHI 467 JAPAN AICHI MED UNIV,INST MED SCI AGING AICHI JAPAN KOHNAN WOMENS COLL,DEPT FOOD & NUTR KOHNAN JAPAN PHARMACIA & UPJOHN INC MILAN ITALY
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 69, anno: 1997,
pagine: 322 - 329
SICI:
0022-3042(1997)69:1<322:AEDNNI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PC12 CELLS; PARKINSONS-DISEASE; IN-VIVO; APOPTOSIS; RAT; 1-METHYL-4-PHENYLPYRIDINIUM; QUANTITATION; SALSOLINOL; ALKALOIDS; ION;
Keywords:
APOPTOSIS; NEUROTOXIN; N-METHYL-(R)-SALSOLINOL; PARKINSONS DISEASE; DOPAMINE NEURON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
W. Maruyama et al., "AN ENDOGENOUS DOPAMINERGIC NEUROTOXIN, N-METHYL-(R)-SALSOLINOL, INDUCES DNA-DAMAGE IN HUMAN DOPAMINERGIC NEUROBLASTOMA SH-SY5Y CELLS", Journal of neurochemistry, 69(1), 1997, pp. 322-329

Abstract

Recently, an endogenous neurotoxin, ethyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline [N-methyl-(R)-salsolinol], was found to elicit parkinsonism in rats with selective depletion of dopamine neurons in the substantia nigra without necrotic tissue reaction. The mechanism of the cell death was examined by detection of DNA damage using a single-cellgel electrophoresis (comet) assay in human dopaminergic neuroblastomaSH-SY5Y cells. Only N-methylsalsolinol was found to induce DNA damage, whereas other catechol isoquinolines, such as (R)-salsolinol, (S)-salsolinol, and 1,2-dimethyl-6,7-dihydroxyisoquinolinium ion, did not. The (R)-enantiomer of N-methylsalsolinol damaged DNA much more profoundly than the (S)-enantiomer. Cycloheximide protected the cells from DNAdamage, suggesting that an apoptotic process may account for the DNA damage, Morphological changes indicating apoptotic cell death were also confirmed. Antioxidants and deprenyl reduced DNA damage, indicating that the damage was initiated by oxidative stress and that neuroprotection by deprenyl may be partially ascribed to its prevention of DNA damage. Apoptosis induced by neurotoxins may be a mechanism underlying the cell death of dopamine neurons in the substantia nigra of Parkinson's disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:39:30