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Titolo:
Postfusional regulation of cleft glutamate concentration during LTP at 'silent synapses'
Autore:
Choi, S; Klingauf, J; Tsien, RW;
Indirizzi:
Stanford Univ, Sch Med, Beckman Ctr, Dept Cellular & Mol Physiol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 & Mol Physiol, Stanford, CA 94305 USA
Titolo Testata:
NATURE NEUROSCIENCE
fascicolo: 4, volume: 3, anno: 2000,
pagine: 330 - 336
SICI:
1097-6256(200004)3:4<330:PROCGC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; CULTURED HIPPOCAMPAL-NEURONS; SYNAPTICALLY RELEASED GLUTAMATE; METHYL-D-ASPARTATE; TRANSPORTER CURRENTS; TRANSMITTER RELEASE; KAINATE RECEPTORS; FUSION PORE; TIME-COURSE; AMPA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Tsien, RW Stanford Univ, Sch Med, Beckman Ctr, Dept Cellular & Mol Physiol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 iol, Stanford, CA 94305 USA
Citazione:
S. Choi et al., "Postfusional regulation of cleft glutamate concentration during LTP at 'silent synapses'", NAT NEUROSC, 3(4), 2000, pp. 330-336

Abstract

'Silent synapses' show responses from high-affinity NMDA receptors (NMDARs) but not low-affinity AMPA receptors (AMPARs), but gain AMPAR responses upon long-term potentiation (LTP). Using the rapidly reversible NMDAR antagonist L-AP5 to assess cleft glutamate concentration ([glu](cleft)), we found that it peaked at <<170 mu M at silent neonatal synapses, but greatly increased after potentiation. Cyclothiazide (CTZ), a potentiator of AMPAR, revealed slowly rising AMPA EPSCs at silent synapses; LTP shortened their rise times. Thus, LTP at silent synapses increased rate-of-rise and peak amplitude of [glu](cleft). Release probability reported by NMDARs remained unchanged during LTP, implying that [glu](cleft) increases arose from immediately presynaptic terminals. Our data suggest that changes in the dynamics of fusion-pore opening contribute to LTP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 14:28:41