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Titolo:
NMDA RECEPTOR-MEDIATED REGULATION OF AMPA RECEPTOR PROPERTIES IN ORGANOTYPIC HIPPOCAMPAL SLICE CULTURES
Autore:
GELLERMAN DM; BI XN; BAUDRY M;
Indirizzi:
UNIV SO CALIF,PROGRAM NEUROSCI,HNB 309 LOS ANGELES CA 90089 UNIV SO CALIF,PROGRAM NEUROSCI LOS ANGELES CA 90089
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 69, anno: 1997,
pagine: 131 - 136
SICI:
0022-3042(1997)69:1<131:NRROAR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; SYNAPTIC PLASTICITY; GLUTAMATE RECEPTORS; CALPAIN INHIBITORS; RAT-BRAIN; BINDING; MEMORY; LTP; ACID; SPECTRIN;
Keywords:
HIPPOCAMPUS; CALPAIN; RECEPTOR; PLASTICITY; GLUTAMATE; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
D.M. Gellerman et al., "NMDA RECEPTOR-MEDIATED REGULATION OF AMPA RECEPTOR PROPERTIES IN ORGANOTYPIC HIPPOCAMPAL SLICE CULTURES", Journal of neurochemistry, 69(1), 1997, pp. 131-136

Abstract

Activation of the calcium-dependent protease calpain has been proposed to be a necessary step in the formation of long-term potentiation (LTP) in the hippocampus, and stimulation of N-methyl-D-aspartate (NMDA)receptors leads to an increase in intracellular calcium concentration, calpain activation, proteolysis of cytoskeletal elements, and modification of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor properties. In the present study, we evaluated the effects of NMDA treatment of cultured hippocampal slices on the properties of AMPA receptors. Cultured hippocampal slices were treated with NMDA (100 mu M) for 15 min and [H-3]AMPA binding to membrane fractions was measured. NMDA-treated slices exhibited an increase in both ''high-affinity''' and ''low-affinity'' [H-3]-AMPA binding, with smaller changesin 6-cyano-7-nitro[H-3] quinoxaline-2,3-dione binding. The increase in [H-3]AMPA binding was significantly reduced by preincubation of cultures with ca[pain inhibitor I or calpeptin (100 mu M). Furthermore, NMDA exposure decreased the number of GluR1 subunits of AMPA receptors detected by an antibody against the C-terminal domain of the subunit inwestern blots and resulted in the formation of a tower molecular weight species detected by an antibody against the N-terminal domain. Botheffects were completely prevented by calpain inhibitors. These results indicate that NMDA receptor activation produces calpain activation and complex modifications of AMPA receptor properties, which could be involved in NMDA receptor-mediated changes in synaptic efficacy.

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Documento generato il 21/01/21 alle ore 03:57:36