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Titolo:
NUCLEAR IMPORT OF HNRNP A1 IS MEDIATED BY A NOVEL CELLULAR COFACTOR RELATED TO KARYOPHERIN-BETA
Autore:
FRIDELL RA; TRUANT R; THORNE L; BENSON RE; CULLEN BR;
Indirizzi:
DUKE UNIV,MED CTR,DEPT GENET DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT GENET DURHAM NC 27710 DUKE UNIV,MED CTR,HOWARD HUGHES MED INST DURHAM NC 27710
Titolo Testata:
Journal of Cell Science
, volume: 110, anno: 1997,
parte:, 11
pagine: 1325 - 1331
SICI:
0021-9533(1997)110:<1325:NIOHAI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORE COMPLEX PROTEIN; TRANSPORT FACTORS; IDENTIFICATION; NUCLEOPORINS; SUBSTRATE; LOCATION; SEQUENCE; BINDING; INTERACTS; DOMAIN;
Keywords:
NUCLEAR TRANSPORT; NLS; HNRNP A1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Fridell et al., "NUCLEAR IMPORT OF HNRNP A1 IS MEDIATED BY A NOVEL CELLULAR COFACTOR RELATED TO KARYOPHERIN-BETA", Journal of Cell Science, 110, 1997, pp. 1325-1331

Abstract

Heterogeneous nuclear ribonucleoprotein Al contains a sequence, termed M9, that functions as a potent nuclear localization signal (NLS) yetbears no similarity to the well-defined basic class of NLSs. Here, wereport the identification of a novel human protein, termed MIP, that binds M9 specifically both in vivo and in vitro yet fails to interact with non-functional M9 point mutants, Of note, the 101 kDa MIP proteinbears significant homology to human karyopherin/importin-beta, a protein known to mediate the function of basic NLSs. The in vitro nuclear import of a protein substrate containing the M9 NLS was found to be dependent on provision of the MIP protein in trans, Cytoplasmic microinjection of a truncated form of MIP that retains the M9 binding site blocked the in vivo nuclear import of a substrate containing the M9 NLS yet failed to affect the import of a similar substrate bearing a basic NLS, These data indicate that nuclear import of hnRNP Al is mediated by a novel cellular import pathway that is distinct from, Set evolutionarily related to, the pathway utilized by basic NLS sequences.

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Documento generato il 02/12/20 alle ore 18:25:43