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Titolo:
Structure of a natural microbial community in a nitroaromatic contaminatedgroundwater is altered during biodegradation of extrinsic, but not intrinsic substrates
Autore:
Wikstrom, P; Hagglund, L; Forsman, M;
Indirizzi:
Def Res Estab, Dept Microbiol, SE-90182 Umea, Sweden Def Res Estab Umea Sweden SE-90182 Dept Microbiol, SE-90182 Umea, Sweden Def Res Estab, Dept Chem, SE-90182 Umea, Sweden Def Res Estab Umea Sweden SE-90182 tab, Dept Chem, SE-90182 Umea, Sweden
Titolo Testata:
MICROBIAL ECOLOGY
fascicolo: 3, volume: 39, anno: 2000,
pagine: 203 - 210
SICI:
0095-3628(200004)39:3<203:SOANMC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMPLIFIED POLYMORPHIC DNA; PSEUDOMONAS; PATHWAYS; GENES; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Forsman, M Def Res Estab, Dept Microbiol, SE-90182 Umea, Sweden Def Res Estab Umea Sweden SE-90182 iol, SE-90182 Umea, Sweden
Citazione:
P. Wikstrom et al., "Structure of a natural microbial community in a nitroaromatic contaminatedgroundwater is altered during biodegradation of extrinsic, but not intrinsic substrates", MICROB ECOL, 39(3), 2000, pp. 203-210

Abstract

This study demonstrates microbial community changes over time in a nitroaromatic-contaminated groundwater upon amendment with hydrocarbons previouslyunknown to the microbial community (extrinsic) and hydrocarbons previouslyknown to the microbial community (intrinsic). Sealed flasks, shaken and incubated at 25 degrees C, containing contaminated groundwater and salts wereamended twice with extrinsic hydrocarbons including phenol, benzoic acid, and naphthalene, and intrinsic hydrocarbons including 2,4-dinitrotoluene (2,4-DNT) and para-nitrotoluene (p-NT). Microbial growth, biodegradation, andcommunity structure changes measured by random amplified polymorphic DNA (RAPD) and quantitative PCR (qPCR) targeting catechol-2,3-dioxygenase (C230)genes were monitored over time. All amended substrates were biodegraded after both substrate amendments except for 2,4-DNT, which was only partially degraded after the second amendment. Unique microbial communities were developed in flasks amended with phenol, benzoic acid, and naphthalene. However, in the flasks amended with intrinsic hydrocarbons the microbial communityremained similar to the unamended control flasks. The relative amount of C230 genes detected by qPCR correlated with the biodegradation of phenol andnaphthalene but not with 2,4-DNT. The results showed that a selection for microorganisms capable of catabolizing extrinsic hydrocarbons naturally andinitially present in the nitroaromatic-contaminated groundwater occurred. However, growth-linked biodegradation of added intrinsic hydrocarbons was not selective.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:44:49